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    High-Contrast Visualization of Upconversion Luminescence in Mice Using Time-Gating Approach

    Access Status
    Fulltext not available
    Authors
    Zheng, X.
    Zhu, X.
    Lu, Y.
    Zhao, J.
    Feng, W.
    Jia, Guohua
    Wang, F.
    Li, F.
    Jin, D.
    Date
    2016
    Type
    Journal Article
    
    Metadata
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    Citation
    Zheng, X. and Zhu, X. and Lu, Y. and Zhao, J. and Feng, W. and Jia, G. and Wang, F. et al. 2016. High-contrast visualization of upconversion luminescence in mice using time-gating approach. Analytical Chemistry. 88 (7): pp. 3449-3454.
    Source Title
    Analytical Chemistry
    DOI
    10.1021/acs.analchem.5b04626
    ISSN
    0003-2700
    School
    Department of Chemistry
    URI
    http://hdl.handle.net/20.500.11937/25271
    Collection
    • Curtin Research Publications
    Abstract

    © 2016 American Chemical Society. Optical imaging through the near-infrared (NIR) window provides deep penetration of light up to several centimeters into biological tissues. Capable of emitting 800 nm luminescence under 980 nm illumination, the recently developed upconversion nanoparticles (UCNPs) suggest a promising optical contrast agent for in vivo bioimaging. However, presently they require high-power lasers to excite when applied to small animals, leading to significant scattering background that limits the detection sensitivity as well as a detrimental thermal effect. In this work, we show that the time-gating approach implementing pulsed illumination from a NIR diode laser and time-delayed imaging synchronized via an optical chopper offers detection sensitivity more than 1 order of magnitude higher than the conventional approach using optical band-pass filters (S/N, 47321/6353 vs 5339/58), when imaging UCNPs injected into Kunming mice. The pulsed laser illumination (70 µs ON in 200 µs period) also reduces the overall thermal accumulation to 35% of that under the continuous-wave mode. Technical details are given on setting up the time-gating unit comprising an optical chopper, a pinhole, and a microscopy eyepiece. Being generally compatible with any camera, this provides a convenient and low cost solution to NIR animal imaging using UCNPs as well as other luminescent probes. (Figure Presented).

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