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    Suppressive subtractive hybridization analysis of Rhipicephalus (Boophilus) microplus larval and adult transcript expression during attachment and feeding

    Access Status
    Fulltext not available
    Authors
    Lew-Tabor, A.
    Moolhuijzen, Paula
    Vance, M.
    Kurscheid, S.
    Valle, M.
    Jarrett, S.
    Minchin, C.
    Jackson, L.
    Jonsson, N.
    Bellgard, M.
    Guerrero, F.
    Date
    2010
    Type
    Journal Article
    
    Metadata
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    Citation
    Lew-Tabor, A. and Moolhuijzen, P. and Vance, M. and Kurscheid, S. and Valle, M. and Jarrett, S. and Minchin, C. et al. 2010. Suppressive subtractive hybridization analysis of Rhipicephalus (Boophilus) microplus larval and adult transcript expression during attachment and feeding. Veterinary Parasitology. 167 (2-4): pp. 304-320.
    Source Title
    Veterinary Parasitology
    DOI
    10.1016/j.vetpar.2009.09.033
    ISSN
    0304-4017
    School
    Centre for Crop Disease Management
    URI
    http://hdl.handle.net/20.500.11937/25811
    Collection
    • Curtin Research Publications
    Abstract

    Ticks, as blood-feeding ectoparasites, affect their hosts both directly and as vectors of viral, bacterial and protozoal diseases. The tick's mode of feeding means it must maintain intimate contact with the host in the face of host defensive responses for a prolonged time. The parasite-host interactions are characterized by the host response and parasite counter-response which result in a highly complex biological system that is barely understood. We conducted transcriptomic analyses utilizing suppressive subtractive hybridization (SSH) to identify transcripts associated with host attachment and feeding of larval, adult female and adult male ticks. Five SSH libraries resulted in 511 clones (assembled into 36 contigs and 90 singletons) from differentially expressed transcripts isolated from unattached frustrated larvae (95), feeding larvae (159), unattached frustrated adult female ticks (68), feeding adult female ticks (95) and male adult ticks (94 clones). Unattached 'frustrated' ticks were held in fabric bags affixed to cattle for up to 24 h to identify genes up-regulated prior to host penetration. Sequence analysis was based on BLAST, Panther, KOG and domain (CDD) analyses to assign functional groups for proteins including: cuticle proteins, enzymes (ATPases), ligand binding (histamine binding), molecular chaperone (prefoldin), nucleic acid binding (ribosomal proteins), putative salivary proteins, serine proteases, stress response (heat shock, glycine rich) and transporters. An additional 63% of all contigs and singletons were novel R. microplus transcripts or predicted proteins of unknown function. Expression was confirmed using quantitative real time PCR analysis of selected transcripts. This is the first comprehensive analysis of the R. microplus transcriptome from multiple stages of ticks and assists to elucidate the molecular events during tick attachment and development. Crown Copyright © 2009.

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    • Comparative microarray analysis of Rhipicephalus (Boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on Bos indicus and Bos taurus cattle
      Rodriguez-Valle, M.; Lew-Tabor, A.; Gondro, C.; Moolhuijzen, Paula; Vance, M.; Guerrero, F.; Bellgard, M.; Jorgensen, W. (2010)
      Background: Rhipicephalus (Boophilus) microplus is an obligate blood feeder which is host specific to cattle. Existing knowledge pertaining to the host or host breed effects on tick transcript expression profiles during ...
    • Differential recognition by tick-resistant cattle of the recombinantly expressed Rhipicephalus microplus serine protease inhibitor-3 (RMS-3)
      Rodriguez-Valle, M.; Vance, M.; Moolhuijzen, Paula; Tao, X.; Lew-Tabor, A. (2012)
      Rhipicephalus microplus is an important bovine ectoparasite, widely distributed in tropical and subtropical regions of the world causing large economic losses to the cattle industry. Its success as an ectoparasite is ...
    • The complexity of Rhipicephalus (Boophilus) microplus genome characterised through detailed analysis of two BAC clones
      Moolhuijzen, Paula; Lew-Tabor, A.; Morgan, J.; Valle, M.; Peterson, D.; Dowd, S.; Guerrero, F.; Bellgard, M.; Appels, R. (2011)
      Background: Rhipicephalus (Boophilus) microplus (Rmi) a major cattle ectoparasite and tick borne disease vector, impacts on animal welfare and industry productivity. In arthropod research there is an absence of a complete ...
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