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    Forensic application of a rapid one-step tetramethylbenzidine-based test for the presumptive trace detection of bloodstains at the crime scene and in the laboratory

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    Access Status
    Open access
    Authors
    De Vittori, E.
    Barni, F.
    Lewis, Simon
    Antonini, G.
    Rapone, C.
    Berti, A.
    Date
    2016
    Type
    Journal Article
    
    Metadata
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    Citation
    De Vittori, E. and Barni, F. and Lewis, S. and Antonini, G. and Rapone, C. and Berti, A. 2016. Forensic application of a rapid one-step tetramethylbenzidine-based test for the presumptive trace detection of bloodstains at the crime scene and in the laboratory. Forensic Chemistry. 2: pp. 63-74.
    Source Title
    Forensic Chemistry
    DOI
    10.1016/j.forc.2016.10.002
    ISSN
    2468-1709
    School
    Department of Chemistry
    URI
    http://hdl.handle.net/20.500.11937/25963
    Collection
    • Curtin Research Publications
    Abstract

    Bloodstains are a widespread kind of biological evidence at the crime scene and one of the most used reagents for the presumptive identification of blood for forensic purposes is tetramethyl-benzidine. We have introduced and validated the tetramethylbenzidine-based Combur3 Test® E (Roche Diagnostics Corporation, Basel, Switzerland), a colorimetric catalytic test based upon the detection of the peroxidase-like activity of the hemoglobin, due to its high sensitivity, easiness of use and capability to maintain the complete structural and morphological integrity of the bloodstain. Analytical performances related to a forensic use of the test and the suitable applicability to the presumptive detection of bloodstains when extremely diluted, aged, mixed with several substances and deposited over a plethora of substrates was reliably proved. In addition, possible positive interferences of the test chemicals on the subsequent Short Tandem Repeats (STRs) DNA typing analyses, especially in Low-Template DNA (LT DNA) conditions, was evaluated. While the Combur3 Test® E showed the same chemical interference drawbacks as other presumptive tests for blood as for the low specificity, we demonstrated that its format and our suggested protocol of use make it appropriate for the forensic presumptive detection of blood, better performing and much easier to use than other analogous presumptive tests and usually compatible with the following STRs DNA typing analyses.

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