Use of fungal transformants expressing ß-glucuronidase activity to detect and measure hyphal biomass in infected plant tissues
MetadataShow full item record
Strains of the tomato pathogen Cladosporium fulvum and the Brassica pathogen Leptosphaeria maculans constitutively expressing Beta-glucuronidase were produced by cotransformation of a hygromycin-encoding vector pAN7-1 and a GUS encoding vector pNOM102. Their Beta-glucuronidase activity was used to detect histochemically the presence of fungal hyphae in host plant tissue. In addition, the Beta-glucuronidase activity of C. fulvum was used to quantify fungal biomass in the cotyledons of near-isogenic lines of tomato containing either no Cf resistance gene, or Cf-3, Cf-5, or Cf-9 resistance genes. Beta-Glucuronidase activity was significantly reduced in incompatible interactions on Cf3, Cf5, and Cf9 plants as compared to the compatible interaction on Cf0. Histochemical staining could also differentiate these interactions. These results demonstrate that the production of Beta-glucuronidase-expressing strains of fungi provides a facile means to detect infection and quantify biomass. Applications of this technique are discussed.
A copy of this item may be available from Professor Richard Oliver
Showing items related by title, author, creator and subject.
Dincer, Tuna (2000)Lactose is the major carbohydrate in milk. The presence of lactose in whey constitutes a significant pollution problem for dairy factories. At the same time, there is an increasing market for high quality crystalline ...
Selective estrogen receptor-beta (SERM-beta) compounds modulate raphe nuclei tryptophan hydroxylase-1 (TPH-1) mRNA expression and cause antidepressant-like effects in the forced swim testClark, J.; Alves, S.; Gundlah, C.; Rocha, B.; Birzin, E.; Cai, S.; Flick, R.; Hayes, E.; Ho, K.; Warrier, Sudha; Pai, L.; Yudkovitz, J.; Fleischer, R.; Colwell, L.; Li, S.; Wilkinson, H.; Schaeffer, J.; Wilkening, R.; Mattingly, E.; Hammond, M.; Rohrer, S. (2012)Estrogen acts through two molecularly distinct receptors termed estrogen receptor alpha (ERa) and estrogen receptor beta (ERß) which bind estradiol with similar affinities and mediate the effects of estrogen throughout ...
Pal, Sebely; Woodford, K.; Kukuljan, S.; Ho, S. (2015)True lactose intolerance (symptoms stemming from lactose malabsorption) is less common than is widely perceived, and should be viewed as just one potential cause of cows’ milk intolerance. There is increasing evidence ...