The three dimensional microstructural network of elastin, collagen and cells in Achilles tendons
MetadataShow full item record
Similar to most biological tissues, the biomechanical and functional characteristics of the Achilles tendon are closely related to its composition and microstructure. It is commonly reported that type I collagen is the predominant component of tendons and is mainly responsible for the tissue's function. Although elastin has been found in varying proportions in other connective tissues, previous studies report that tendons contain very small quantities of elastin. However, the morphology of and the microstructural relationship among the elastic fibres, collagen and cells in tendon tissue have not been well examined. We hypothesize the elastic fibres, as another fibrillar component in the extracellular matrix, have a unique role in mechanical functions and microstructural arrangement in Achilles tendons. Using confocal and Second Harmonic Generation (SHG) imaging techniques, this study examined the 3-dimensional microstructure of the collagen, elastin and cells in the mid-portion of hydrated rabbit Achilles tendons. It has been shown that elastic fibres present a close connection with the tenocytes. The close relationship of the three components has been revealed as a distinct, integrated and complex microstructural network. Notably, a "spiral" structure within fibril bundles in Achilles tendons was observed in some samples in specialized regions. This study substantiates the hierarchical system of the spatial microstructure of tendon, including the mapping of collagen, elastin and tenocytes, with 3-dimensional confocal images.
Showing items related by title, author, creator and subject.
High-resolution study of the 3D collagen fibrillary matrix of Achilles tendons without tissue labelling and dehydratingWu, Jian-Ping; Swift, B.; Becker, Thomas; Squelch, Andrew; Wang, A.; Zheng, Y.; Zhao, X.; Xu, J.; Xue, W.; Zheng, M.; Lloyd, D.; Kirk, Brett (2017)Â© 2017 The Authors Journal of Microscopy Â© 2017 Royal Microscopical Society Knowledge of the collagen structure of an Achilles tendon is critical to comprehend the physiology, biomechanics, homeostasis and remodelling ...
High-resolution study of the 3D collagen fibrillary matrix of Achilles tendons without tissue labelling and dehydratingWu, J.; Swift, B.; Becker, Thomas; Squelch, Andrew; Wang, A.; Zheng, Y.; Zhao, X.; Xu, J.; Xue, W.; Zheng, M.; Lloyd, D.; Kirk, Brett (2017)Knowledge of the collagen structure of an Achilles tendon is critical to comprehend the physiology, biomechanics, homeostasis and remodelling of the tissue. Despite intensive studies, there are still uncertainties regarding ...
Pang, Xin (2017)Utilising confocal and second harmonic generation microscopy, this PhD project developed a 3D imaging technique and studied the cell morphology and microstructure of the collagen fibrils and elastic fibres of healthy ...