Rapid determination of Papaver somniferum alkaloids in process streams using monolithic column high-performance liquid chromatography with chemiluminescence detection
MetadataShow full item record
We have combined high-performance liquid chromatography (HPLC) separations using a monolithic column with acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection in a rapid and highly sensitive method to monitor the process of extracting opiate alkaloids from Papaver somniferum. Due to the high flow rates allowed with the monolithic column and the inherent selectivity of the chemiluminescence reactions, the four predominant alkaloids - morphine, codeine, oripavine and thebaine - were determined in less than 2 min. The results obtained with numerous process samples compared favourable with those of the standard HPLC methodology. Limits of detection were 11010 M, 51010 M, 51010M and 1109 M, for morphine, codeine, oripavine and thebaine, respectively.
Costin, Jason and Lewis, Simon and Percell, Stuart and Waddell, Lucy and Francis, Paul and Barnett, Neil (2007) Rapid determination of Papaver somniferum alkaloids in process streams using monolithic column high-performance liquid chromatography with chemiluminescence detection, Analytica Chimica Acta 597(1):19-23.
The link to this article is: http://dx.doi.org/10.1016/j.aca.2007.06.044
Copyright 2007 Elsevier B.V. All rights reserved.
Showing items related by title, author, creator and subject.
The Determination of Morphine in the Larvae of Calliphora stygia using Flow Injection Analysis and HPLC with Chemiluminescence DetectionGunn, J.; Shelley, C.; Lewis, Simon; Toop, T.; Archer, M. (2006)Selective determination of morphine in the larvae of Calliphora stygia (Fabricius) (Diptera: Calliphoridae) using acidic potassium permanganate chemiluminescence detection coupled with flow injection analysis and ...
Zhang, Xihong; Hao, Hong; Li, C. (2016)Although precast concrete segmental columns have been more and more widely used in constructions, there is a lack of study on its behaviour under dynamic loading especially impact loading. In this paper, the fundamental ...
Li, J.; Hao, Hong; Wu, C. (2017)Constructions with precast technology have seen a fast development over the past several decades. Despite advantages including short construction period, better quality control, less environmental and traffic impact, a ...