Ion-transfer electrochemistry of rat amylin at the water–organogel microinterface array and its selective detection in a protein mixture
MetadataShow full item record
The behaviour of proteins and polypeptides at electrified aqueous-organic interfaces is of benefit in label-free detection strategies. In this work, rat amylin (or islet amyloid polypeptide) was studied at the interface formed between liquid aqueous and gelled organic phases. Amylin is a polypeptide that is co-secreted with insulin from islet beta-cells and is implicated in fibril formation. In this study, rat amylin was used, which does not undergo aggregation. The polypeptide was found to undergo an interfacial transfer process, from water to the gelled organic phase under applied potential stimulation. The voltammetry exhibited steady-state forward and peak-shaped reverse voltammograms, which are consistent with a diffusion-controlled water-to-organic transfer and a thin-film stripping or desorptive back-transfer. The diffusion-controlled forward current was greater when amylin was present in an acidic aqueous phase than when it was present in aqueous phase at physiological pH, reflecting the greater charge on the polypeptide under acidic conditions. The amylin transfer current was concentration-dependent over the range 2 - 10 µM, at both acidic and physiological pH. At physiological pH, amylin was selectively detected in the presence of a protein mixture, illustrating the bioanalytical possibilities for this electrochemical behaviour.
Showing items related by title, author, creator and subject.
Size exclusion chromatography as a tool for natural organic matter characterisation in drinking water treatmentAllpike, Bradley (2008)Natural organic matter (NOM), ubiquitous in natural water sources, is generated by biogeochemical processes in both the water body and in the surrounding watershed, as well as from the contribution of organic compounds ...
Electrochemical ion transfer across liquid/liquid interfaces confined within solid-state micropore arrays – simulations and experimentsStrutwolf, J.; Scanlon, M.; Arrigan, Damien (2009)Miniaturised liquid/liquid interfaces provide benefits for bioanalytical detection with electrochemicalmethods. In this work, microporous silicon membranes which can be used for interface miniaturisationwere characterized ...
Scanlon, M.; Strutwolf, J.; Arrigan, Damien (2010)The behaviour of two biological macromolecules, bovine pancreatic insulin and hen-egg-whitelysozyme (HEWL), at aqueous-organogel interfaces confined within an array of solid-state membrane micropores was investigated via ...