Gene identification in the obligate fungal pathogen Blumeria graminis by expressed sequence tag analysis
Access Status
Authors
Date
2001Type
Metadata
Show full item recordCitation
Faculty
Remarks
A copy of this item may be available from Professor Richard Oliver
Email: Richard.oliver@curtin.edu.au
Collection
Abstract
Powdery mildew of barley is caused by the obligate fungal pathogen Blumeria graminis f. sp. hordei. Haploid conidia of B. graminis, landing on the barley leaf, germinate to form first a primary germ tube and then an appressorial germ tube. The appressorial germ tube differentiates into a mature appressorium from which direct penetration of host epidermis occurs. Here we present data on 4908 expressed sequence tags obtained from B. graminis conidia. The combined sequences represent 2676 clones describing 1669 individual genes. Comparison with sequences from other pathogenic and nonpathogenic fungi defines hypotheses on the genes required for pathogenicity and growth on the host. The putative roles of some of the identified genes are discussed.
Related items
Showing items related by title, author, creator and subject.
-
Lu, X.; Kracher, B.; Saur, I.; Bauer, S.; Ellwood, Simon; Wise, R.; Yaeno, T.; Maekawa, T.; Schulze-Lefert, P. (2016)© 2016, National Academy of Sciences. All rights reserved. Disease-resistance genes encoding intracellular nucleotide-binding domain and leucine-rich repeat proteins (NLRs) are key components of the plant innate immune ...
-
Karaoglu, H.; Lee, Crystal; Park, R. (2013)We evaluated the abundance and nature of simple sequence repeats (SSRs) in the causal agent of stem rust of wheat, Puccinia graminis f. sp. tritici, in order to characterize and develop SSR markers. A strategy was tested ...
-
Bindslev, L.; Oliver, Richard; Johansen, B. (2002)PCR and DNA sequence analysis have become standard tools for identification, detection and phylogenetic analysis of fungi. A large number of species are incapable of growth in the laboratory, making the preparation of ...