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    Complementation of the Magnaporthe grisea cpkA mutation by the Blumeria graminis PKA-c gene: functional genetic analysis of an obligate plant pathogen

    Access Status
    Fulltext not available
    Authors
    Bindslev, L.
    Kershaw, M.
    Talbot, N.
    Oliver, Richard
    Date
    2001
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    BINDSLEV L, KERSHAW MJ, TALBOT NJ, AND OLIVER RP (2001) Complementation of the Magnaporthe grisea ΔcpkA mutation by the Blumeria graminis PKA-c gene: functional genetic analysis of an obligate plant pathogen. Molecular Plant Microbe Interactions 14 1368-1375
    DOI
    10.1094/MPMI.2001.14.12.1368
    Faculty
    Department of Environmental & Agriculture
    School of Agriculture and Environment
    Faculty of Science and Engineering
    Remarks

    A copy of this item may be available from Professor Richard Oliver

    Email: Richard.oliver@curtin.edu.au

    URI
    http://hdl.handle.net/20.500.11937/27787
    Collection
    • Curtin Research Publications
    Abstract

    Obligate plant-pathogenic fungi have proved extremely difficult to characterize with molecular genetics because they cannot be cultured away from host plants and only can be manipulated experimentally in limited circumstances. Previously, in order to characterize signal transduction processes during infection-related development of the powdery mildew fungus Blumeria graminis (syn. Erysiphe graminis) f. sp. hordei, we described a gene similar to the catalytic subunit of cyclic AMP-dependent protein kinase A (here renamed Bka1). Functional characterization of this gene has been achieved by expression in a ΔcpkA mutant of the nonobligate pathogen Magnaporthe grisea. This nonpathogenic M. grisea ΔcpkA mutant displays delayed and incomplete appressorium development, suggesting a role for PKA-c in the signal transduction processes that control the maturation of infection cells. Transformation of the ΔcpkA mutant with the mildew Bka1 open reading frame, controlled by the M. grisea MPG1 promoter, restored pathogenicity and appressorium maturation kinetics. The results provide, to our knowledge, the first functional genetic analysis of pathogenicity in an obligate pathogen and highlight the remarkable conservation of signaling components regulating infection-related development in pathogenic fungi.

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