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    A parametric study of a monolithic microfluidic system for on-chip biomolecular separation

    Access Status
    Fulltext not available
    Authors
    Chan, A.
    Danquah, Michael
    Agyei, D.
    Hartley, P.
    Zhu, Y.
    Date
    2014
    Type
    Journal Article
    
    Metadata
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    Citation
    Chan, A. and Danquah, M. and Agyei, D. and Hartley, P. and Zhu, Y. 2014. A parametric study of a monolithic microfluidic system for on-chip biomolecular separation. Separation Science and Technology. 49 (6): pp. 854-860.
    Source Title
    Separation Science and Technology
    DOI
    10.1080/01496395.2013.872144
    ISSN
    0149-6395
    School
    Department of Chemical Engineering
    URI
    http://hdl.handle.net/20.500.11937/28015
    Collection
    • Curtin Research Publications
    Abstract

    A microfabricated poly(dimethylsiloxane) (PDMS) chip containing channel filled with polymer monolith has been developed for on-chip biomolecule separation. Methacrylate monolithic polymers were prepared by photo-initiated polymerization within the channel to serve as a continuous stationary phase. The monolithic polymer was functionalized with a weak anion-exchange ligand, and key parameters affecting the binding characteristics of the system were investigated. The total binding capacity was unaffected by the flow rate of the mobile phase but varied significantly with changes in ionic strength and pH of the binding buffer. The binding capacity decreased with increasing buffer ionic strength, and this is due to the limited available binding sites for protein adsorption resulting from cationic shielding effect. Similarly, the binding capacity decreased with decreasing buffer pH towards the isoelectric point of the protein. A protein mixture, BSA and ovalbumin, was used to illustrate the capacity of the methacrylate-based microfluidic chip for rapid biomolecule separation.

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