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    Identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, Amphiprion mccullochi

    Access Status
    Fulltext not available
    Authors
    van der Meer, M.
    Gardner, M.
    Hobbs, Jean-Paul
    Jones, G.
    van Herwerden, L.
    Date
    2012
    Type
    Journal Article
    
    Metadata
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    Citation
    van der Meer, M. and Gardner, M. and Hobbs, J.-P. and Jones, G. and van Herwerden, L. 2012. Identification of seventeen microsatellite markers for conservation genetic studies of the endemic anemonefish, Amphiprion mccullochi. Conservation Genetics Resources. 4 (2): pp. 247-250.
    Source Title
    Conservation Genetics Resources
    DOI
    10.1007/s12686-011-9517-1
    ISSN
    18777252
    URI
    http://hdl.handle.net/20.500.11937/28041
    Collection
    • Curtin Research Publications
    Abstract

    Endemic species at remote islands have a high risk of extinction because they often exhibit ecological, biological and genetic traits that make them particularly vulnerable to disturbances. McCulloch’s anemonefish (Amphiprion mccullochi) is endemic to a few oceanic reefs off Australia’s east coast and is an habitat specialist. Using 454 shotgun sequencing, we developed and evaluated primers for seventeen independent microsatellite loci to reveal gene flow, population genetic structure and genetic diversity across three isolated reefs. Observed and expected heterozygosities ranged from 0.556 to 1 and 0.543 to 0.907, respectively, in 30 Lord Howe Island individuals. When cross tested with the close relative, Amphiprion akindynos, amplification was successful with high levels of polymorphism. These loci will therefore be useful in studies of A. mccullochi, A. akindynos and possibly other closely related anemonefish.

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