Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Structure and energy of a DNA dodecamer under tensile load

    19512_19512.pdf (803.9Kb)
    Access Status
    Open access
    Authors
    Piana, Stefano
    Date
    2005
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Piana, Stefano. 2005. Structure and energy of a DNA dodecamer under tensile load. Nucleic Acids Research 33: 7029-7038.
    Source Title
    Nucleic Acids Research
    DOI
    10.1093/nar/gki1010
    Faculty
    Department of Applied Chemistry
    Division of Engineering, Science and Computing
    Faculty of Science
    Remarks

    This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Nucleic Acids Research following peer review. The definitive publisher-authenticated version is available online at: http://dx.doi.org/doi:10.1093/nar/gki1010

    URI
    http://hdl.handle.net/20.500.11937/28262
    Collection
    • Curtin Research Publications
    Abstract

    In the last decade, methods to study single DNA molecules under tensile load have been developed. These experiments measure the force required to stretch and melt the double helix and provide insights into the structural stability of DNA. However, it is not easy to directly relate the shape of the force curve to the structural changes that occur in the double helix under tensile load. Here, state-of-the-art computer simulations of short DNA sequences are preformed to provide an atomistic description of the stretching of the DNA double helix. These calculations show that for extensions larger that ~25% the DNA undergoes a structural transformation and a few base pairs are lost from both the terminal and central part of the helix. This locally melted DNA duplex is stable and can be extended up to ~50-60% of the equilibrium length at a constant force. It is concluded that melting under tension cannot be modeled as a simple two-state process. Finally, the important role of the cantilever stiffness in determining the shape of the force- extension curve and the most probable rupture force is discussed.

    Related items

    Showing items related by title, author, creator and subject.

    • Molecular analysis of genes encoding resistance to Cationic Biocides in staphylococci
      Morgan, Dale (2007)
      Bacterial resistance to non-antibiotic agents is being increasingly studied. Plasmid-mediated resistance to cationic agents, which are important biocides, has been described in antibiotic-resistant Staphylococcus aureus. ...
    • Surface movement in water of splendipherin, the aquatic male sex pheromone of the tree frog Litoria splendida
      Perriman, A.; Apponyi, M.; Buntine, Mark; Jackway, R.; Rutland, M.; White, J.; Bowie, J. (2008)
      The aquatic sex pheromone splendipherin (GLVSSIGKALGGLLADVVKSKGQPA-OH) of the male green tree frog Litoria splendida movesacross the surface of water to reach the female. Surface pressure and X-ray reflectometry measurements ...
    • The Effect of Distribution for a Moving Force
      Reda, A.; Forbes, Gareth (2011)
      Many types of slender or thin walled structures experience forces which traverse across them. For example: vehicles passing over a bridge, overhead crane operations and liquid "slug" movement in spanning pipelines. This ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.