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    Divergent LIN28-mRNA associations result in translational suppression upon the initiation of differentiation

    204824_118962_Lareu_NuclAcidRes_2014__in_print_.pdf (2.795Mb)
    Access Status
    Open access
    Authors
    Tan, S.
    Altschuler, G.
    Zhao, T.
    Ang, H.
    Yang, H.
    Lim, B.
    Vardy, L.
    Hide, W.
    Thomson, A.
    Lareu, Ricky R.
    Date
    2014
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Tan, S. and Altschuler, G. and Zhao, T. and Ang, H. and Yang, H. and Lim, B. and Vardy, L. et al. 2014. Divergent LIN28-mRNA associations result in translational suppression upon the initiation of differentiation. Nucleic Acids Research. 42 (12): pp. 7997-8007.
    Source Title
    Nucleic Acids Research
    DOI
    10.1093/nar/gku430
    ISSN
    0305-1048
    School
    School of Pharmacy
    Remarks

    This article is published under the Open Access publishing model and distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/ Please refer to the licence to obtain terms for any further reuse or distribution of this work.

    URI
    http://hdl.handle.net/20.500.11937/29802
    Collection
    • Curtin Research Publications
    Abstract

    LIN28 function is fundamental to the activity and behavior of human embryonic stem cells (hESCs) and induced pluripotent stem cells. Its main roles in these cell types are the regulation of translational efficiency and let-7 miRNA maturation. However, LIN28-associated mRNA cargo shifting and resultant regulation of translational efficiency upon the initiation of differentiation remain unknown. An RNA-immunoprecipitation and microarray analysis protocol, eRIP, that has high specificity and sensitivity was developed to test endogenous LIN28-associated mRNA cargo shifting. A combined eRIP and polysome analysis of early stage differentiation of hESCs with two distinct differentiation cues revealed close similarities between the dynamics of LIN28 association and translational modulation of genes involved in the Wnt signaling, cell cycle, RNA metabolism and proteasomal pathways. Our data demonstrate that change in translational efficiency is a major contributor to early stages of differentiation of hESCs, in which LIN28 plays a central role. This implies that eRIP analysis of LIN28-associated RNA cargoes may be used for rapid functional quality control of pluripotent stem cells under manufacture for therapeutic applications.

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