Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Subcellular biochemical investigation of purkinje neurons using synchrotron radiation fourier transform infrared spectroscopic imaging with a focal plane array detector

    Access Status
    Fulltext not available
    Authors
    Hackett, Mark
    Borondics, F.
    Brown, D.
    Hirschmugl, C.
    Smith, S.
    Paterson, P.
    Nichol, H.
    Pickering, I.
    George, G.
    Date
    2013
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Hackett, M. and Borondics, F. and Brown, D. and Hirschmugl, C. and Smith, S. and Paterson, P. and Nichol, H. et al. 2013. Subcellular biochemical investigation of purkinje neurons using synchrotron radiation fourier transform infrared spectroscopic imaging with a focal plane array detector. ACS Chemical Neuroscience. 4 (7): pp. 1071-1080.
    Source Title
    ACS Chemical Neuroscience
    DOI
    10.1021/cn4000346
    School
    Department of Chemistry
    URI
    http://hdl.handle.net/20.500.11937/30173
    Collection
    • Curtin Research Publications
    Abstract

    Coupling Fourier transform infrared spectroscopy with focal plane array detectors at synchrotron radiation sources (SR-FTIR-FPA) has provided a rapid method to simultaneously image numerous biochemical markers in situ at diffraction limited resolution. Since cells and nuclei are well resolved at this spatial resolution, a direct comparison can be made between FTIR functional group images and the histology of the same section. To allow histological analysis of the same section analyzed with infrared imaging, unfixed air-dried tissue sections are typically fixed (after infrared spectroscopic analysis is completed) via immersion fixation. This post fixation process is essential to allow histological staining of the tissue section. Although immersion fixation is a common practice in this filed, the initial rehydration of the dehydrated unfixed tissue can result in distortion of subcellular morphology and confound correlation between infrared images and histology. In this study, vapor fixation, a common choice in other research fields where postfixation of unfixed tissue sections is required, was employed in place of immersion fixation post spectroscopic analysis. This method provided more accurate histology with reduced distortions as the dehydrated tissue section is fixed in vapor rather than during rehydration in an aqueous fixation medium. With this approach, accurate correlation between infrared images and histology of the same section revealed that Purkinje neurons in the cerebellum are rich in cytosolic proteins and not depleted as once thought. In addition, we provide the first direct evidence of intracellular lactate within Purkinje neurons. This highlights the significant potential for future applications of SR-FTIR-FPA imaging to investigate cellular lactate under conditions of altered metabolic demand such as increased brain activity and hypoxia or ischemia. © 2013 American Chemical Society.

    Related items

    Showing items related by title, author, creator and subject.

    • Chemical alterations to murine brain tissue induced by formalin fixation: Implications for biospectroscopic imaging and mapping studies of disease pathogenesis
      Hackett, Mark; McQuillan, J.; El-Assaad, F.; Aitken, J.; Levina, A.; Cohen, D.; Siegele, R.; Carter, E.; Grau, G.; Hunt, N.; Lay, P. (2011)
      Understanding biochemical mechanisms and changes associated with disease conditions and, therefore, development of improved clinical treatments, is relying increasingly on various biochemical mapping and imaging techniques ...
    • A novel multi-modal platform to image molecular and elemental alterations in ischemic stroke
      Caine, S.; Hackett, Mark; Hou, H.; Kumar, S.; Maley, J.; Ivanishvili, Z.; Suen, B.; Szmigielski, A.; Jiang, Z.; Sylvain, N.; Nichol, H.; Kelly, M. (2016)
      © 2016 Elsevier Inc.Stroke is a major global health problem, with the prevalence and economic burden predicted to increase due to aging populations in western society. Following stroke, numerous biochemical alterations ...
    • Novel bio-spectroscopic imaging reveals disturbed protein homeostasis and thiol redox with protein aggregation prior to hippocampal CA1 pyramidal neuron death induced by global brain ischemia in the rat
      Hackett, Mark; Smith, S.; Caine, S.; Nichol, H.; George, G.; Pickering, I.; Paterson, P. (2015)
      Global brain ischemia resulting from cardiac arrest and cardiac surgery can lead to permanent brain damage and mental impairment. A clinical hallmark of global brain ischemia is delayed neurodegeneration, particularly ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.