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    Rs1888747 polymorphism in the FRMD3 gene, gene and protein expression: Role in diabetic kidney disease

    238341_238341.pdf (1.399Mb)
    Access Status
    Open access
    Authors
    Buffon, M.
    Carpena, M.
    Sortica, D.
    Santer, A.
    Carlessi, Rodrigo
    De Souza, B.
    Edelweiss, M.
    Berger, M.
    Crispim, D.
    Canani, L.
    Date
    2016
    Type
    Journal Article
    
    Metadata
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    Citation
    Buffon, M. and Carpena, M. and Sortica, D. and Santer, A. and Carlessi, R. and De Souza, B. and Edelweiss, M. et al. 2016. Rs1888747 polymorphism in the FRMD3 gene, gene and protein expression: Role in diabetic kidney disease. Diabetology and Metabolic Syndrome. 8 (3): pp. 1-10.
    Source Title
    Diabetology and Metabolic Syndrome
    DOI
    10.1186/s13098-015-0121-5
    School
    School of Biomedical Sciences
    Remarks

    This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    URI
    http://hdl.handle.net/20.500.11937/30175
    Collection
    • Curtin Research Publications
    Abstract

    © 2016 Buffon et al. Background: We carried out a case-control study in patients with type 2 diabetes mellitus (T2DM) to evaluate the association between seven single nucleotide polymorphisms (SNPs) previously described to be linked to diabetic kidney disease (DKD) in type 1 diabetes mellitus (T1DM). Additionally, we evaluated gene and protein expression related to the polymorphism associated with DKD. Methods: The association study included 1098 T2DM patients (718 with DKD and 380 without DKD). Out of the 13 polymorphisms associated with DKD in a previous study with T1DM, seven were chosen for evaluation in this sample: rs1888747, rs9521445, rs39075, rs451041, rs1041466, rs1411766 and rs6492208. The expression study included 91 patients who underwent nephrectomy. Gene expression was assessed by RT-qPCR and protein expression in kidney samples was quantified by western blot and it localization by immunohistochemistry. Results: The C/C genotype of rs1888747 SNP was associated with protection for DKD (OR = 0.6, 95 % CI 0.3-0.9; P = 0.022). None of the other SNPs were associated with DKD. rs1888747 is located near FRMD3 gene. Therefore, FRMD3 gene and protein expression were evaluated in human kidney tissue according to rs1888747 genotypes. Gene and protein expression were similar in subjects homozygous for the C allele and in those carrying the G allele. Conclusions: Replication of the association between rs1888747 SNP and DKD in a different population suggests that this link is not the result of chance. rs1888747 SNP is located at the FRMD3 gene, which is expressed in human kidney. Therefore, this gene is a candidate gene for DKD. However, in this study, no rs1888747 genotype or specific allele effect on gene and/or protein expression of the FRMD3 gene was demonstrated.

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