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    Alginate bead-encapsulated PEDF induces ectopic bone formation in vivo in the absence of co-administered mesenchymal stem cells.

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    Authors
    Elahy, M.
    Doschak, M.
    Hughes, J.
    Baindur-Hudson, S.
    Dass, Crispin
    Date
    2015
    Type
    Journal Article
    
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    Citation
    Elahy, M. and Doschak, M. and Hughes, J. and Baindur-Hudson, S. and Dass, C. 2015. Alginate bead-encapsulated PEDF induces ectopic bone formation in vivo in the absence of co-administered mesenchymal stem cells.. Curr Drug Targets.
    Source Title
    Curr Drug Targets
    School
    School of Pharmacy
    URI
    http://hdl.handle.net/20.500.11937/30515
    Collection
    • Curtin Research Publications
    Abstract

    Bone defects can be severely debilitating and reduce quality of life. Osteoregeneration can alleviate some of the complications in bony defects. For therapeutic use in future, a single factor that can cause potent bone regeneration is highly preferred as it will be more cost-effective, any off-target effects will be more easily monitored and potentially managed, and for ease of administration which would lead to better patient compliance and satisfaction. We demonstrate that pigment epithelium-derived factor (PEDF), one such factor that is known to be potent against angiogenesis, promotes osteoblastogenesis in mesenchymal stem cells in vitro, but does not need co-encapsulation of cells in alginate bead scaffolds for osteogeneration in vivo. Osteogenic differentiation by PEDF in vitro was confirmed with immunoblotting and immunocytochemical staining for bone markers (alkaline phosphatase, osteocalcin, osteopontin, collagen I), calcified mineral deposition, and assay for alkaline phosphatase activity. PEDF-mediated bone formation in a muscle pocket in vivo model was confirmed by microcomputed tomography (microCT), histology (haematoxylin and eosin, Alcian blue staining), immunostaining for bone markers and for collagen I-processing proteins (heat shock protein 47 and membrane type I matrix metalloproteinase). PEDF therefore presents itself as a promising biological for osteogeneration.

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