Adsorptive stripping voltammetry of hen-egg-white-lysozyme via adsorption-desorption at an array of liquid-liquid microinterfaces
MetadataShow full item record
Electrochemical adsorption and voltammetry of hen-egg-white-lysozyme (HEWL) was studied at an array of microinterfaces between two immiscible electrolyte solutions (µITIES). Adsorption of the protein was achieved at an optimal applied potential of 0.95 V, after which it was desorbed by a voltammetric scan to lower potentials. The voltammetric peak recorded during the desorption scan was dependent on the adsorption time and on the aqueous phase concentration of HEWL. The slow approach to saturation or equilibrium indicated that protein reorganization at the interface was the rate-determining step and not diffusion to the interface. For higher concentrations and longer adsorption times, a HEWL multilayer surface coverage of 550 pmol cm-2 was formed, on the basis of the assumption that a single monolayer corresponded to a surface coverage of 13 pmol cm-2. Implementation of adsorption followed by voltammetric detection as an adsorptive stripping voltammetric approach to HEWL detection demonstrated a linear dynamic range of 0.05-1 µM and a limit of detection of 0.03 µM, for 5 min preconcentration in unstirred solution; this is a more than 10-fold improvement over previous HEWL detection methods at the ITIES. These results provide the basis for a new analytical approach for label-free protein detection based on adsorptive stripping voltammetry.
Showing items related by title, author, creator and subject.
Electrochemical behaviour of hen-egg-white lysozyme at the polarised water/1,2-dichloroethane interfaceScanlon, M.; Jennings, E.; Arrigan, Damien (2009)The electrochemical behaviour of hen-egg-white lysozyme (HEWL) was studied at the polarizedwater/1,2-dichloroethane interface. The voltammetric ion-transfer response was found to bedependent on the pH and ionic strength ...
Investigation of a solvent-cast organogel to form a liquid-gel microinterface array for electrochemical detection of lysozymeFelisilda, B.; Alvarez De Eulate, Eva; Arrigan, Damien (2015)Ion transfer at aqueous-organogel interfaces enables the non-redox detection of ions and ionisable species by voltammetry. In this study, a non-thermal method for preparation of an organogel was employed and used for the ...
Electrochemical signature of hen egg white lysozyme at the glycerol-modified liquid-liquid interfaceAusten, Benjamin; Arrigan, Damien (2016)© 2016 Elsevier Ltd. Electrochemical characterization of hen egg white lysozyme (HEWL) at a glycerol-modified interface between two immiscible electrolyte solutions (ITIES) was conducted using a microporous silicon ...