Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Genetic diversity among pandemic 2009 influenza viruses isolated from a transmission chain

    Access Status
    Fulltext not available
    Authors
    Fordyce, S.
    Bragstad, K.
    Pedersen, S.
    Jensen, T.
    Gahrn-Hansen, B.
    Daniels, R.
    Hay, A.
    Kampmann, M.
    Bruhn, C.
    Moreno-Mayar, V.
    Avila-Arcos, M.
    Gilbert, Thomas
    Nielsen, L.
    Date
    2013
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Fordyce, S. and Bragstad, K. and Pedersen, S. and Jensen, T. and Gahrn-Hansen, B. and Daniels, R. and Hay, A. et al. 2013. Genetic diversity among pandemic 2009 influenza viruses isolated from a transmission chain. Virology Journal. 10: pp. 116-1-116-9.
    Source Title
    Virology Journal
    Additional URLs
    http://www.biomedcentral.com/content/pdf/1743-422X-10-116.pdf
    ISSN
    1743-422X
    URI
    http://hdl.handle.net/20.500.11937/30834
    Collection
    • Curtin Research Publications
    Abstract

    Background: Influenza viruses such as swine-origin influenza A(H1N1) virus (A(H1N1)pdm09) generate genetic diversity due to the high error rate of their RNA polymerase, often resulting in mixed genotype populations (intrahost variants) within a single infection. This variation helps influenza to rapidly respond to selection pressures, such as those imposed by the immunological host response and antiviral therapy. We have applied deep sequencing to characterize influenza intra-host variation in a transmission chain consisting of three cases due to oseltamivirsensitive viruses, and one derived oseltamivir-resistant case. Methods: Following detection of the A(H1N1)pdm09 infections, we deep-sequenced the complete NA gene from two of the oseltamivir-sensitive virus-infected cases, and all eight gene segments of the viruses causing the remaining two cases.Results: No evidence for the resistance-causing mutation (resulting in NA H275Y substitution) was observed in the oseltamivir-sensitive cases. Furthermore, deep sequencing revealed a subpopulation of oseltamivir-sensitive viruses in the case carrying resistant viruses. We detected higher levels of intra-host variation in the case carrying oseltamivir-resistant viruses than in those infected with oseltamivir-sensitive viruses. Conclusions: Oseltamivir-resistance was only detected after prophylaxis with oseltamivir, suggesting that the mutation was selected for as a result of antiviral intervention. The persisting oseltamivir-sensitive virus population in the case carrying resistant viruses suggests either that a small proportion survive the treatment, or that the oseltamivir-sensitive virus rapidly re-establishes itself in the virus population after the bottleneck. Moreover, the increased intra-host variation in the oseltamivir-resistant case is consistent with the hypothesis that the population diversity of a RNA virus can increase rapidly following a population bottleneck.

    Related items

    Showing items related by title, author, creator and subject.

    • Evidence for an ancient aquatic origin of the RNA viral order Articulavirales
      Petrone, M.E.; Parry, R.; Mifsud, J.C.O.; Van Brussel, K.; Vorhees, I.; Richards, Zoe ; Holmes, E.C. (2023)
      The emergence of previously unknown disease-causing viruses in mammals is in part the result of a long-term evolutionary process. Reconstructing the deep phylogenetic histories of viruses helps identify major evolutionary ...
    • Genome-wide genetic investigation of serological measures of common infections
      Rubicz, R.; Yolken, R.; Drigalenko, E.; Carless, M.; Dyer, T.; Kent, J.; Curran, J.; Johnson, M.; Cole, S.; Fowler, S.; Arya, R.; Puppala, S.; Almasy, L.; Moses, Eric; Kraig, E.; Duggirala, R.; Blangero, J.; Leach, C.; Göring, H. (2015)
      Populations and individuals differ in susceptibility to infections because of a number of factors, including host genetic variation. We previously demonstrated that differences in antibody titer, which reflect infection ...
    • Comparison between Nasal Swabs and Nasopharyngeal Aspirates for, and Effect of Time in Transit on, Isolation of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, and Moraxella catarrhalis
      Carville, K.; Bowman, J.; Lehmann, Deborah; Riley, T. (2007)
      pmc logo image Logo of jcm Note: Performing your original search, comparison nasal swabs lehmann, in PubMed Central will retrieve 7 citations. Journal List > J Clin Microbiol > v.45(1); Jan 2007 Abstract ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.