Growth in the presence of sucrose may decrease attachment of some oral bacteria to abiotic surfaces
MetadataShow full item record
Synthesis of cell-bound glucan from dietary sucrose by oral pathogenic bacteria may influence bacterial cell surface properties and colonization of surfaces. This study investigated the effects of the addition of 2 % sucrose to culture medium on cell surface properties (hydrophobicity, charge, and auto-aggregation) and colonization activities (attachment and biofilm formation) on three abiotic surfaces (hydroxyapatite, glass, and stainless steel) of two Streptococcus mutans strains, one Streptococcus salivarius strain, one Streptococcus mitis strain, and one Actinomyces naeslundii strain. The results showed that the additional sucrose reduced the hydrophobicity of three strains (44-62 %) and increased that of one strain (31 %). Cellular aggregation of one strain was decreased (13 %) and that of another increased (21 %). No change in the surface charge of strains was apparent. Additional sucrose also inhibited the attachment of three strains (0.6-1.3 log CFU cm−2) and enhanced that of one strain (0.5-1.3 log CFU cm−2) to glass and stainless steel. The attachment of two strains to hydroxyapatite was reduced (0.9-1.3 log CFU cm−2). Biofilm formation by four strains was enhanced on all surfaces (0.4-1 log CFU cm−2). No relationship between changes in cell surface properties and changes in colonization activities was apparent. Sucrose does not always enhance oral bacterial colonization of abiotic surfaces.
Showing items related by title, author, creator and subject.
Attachment of Salmonella strains to a plant cell wall model is modulated by surface characteristics and not by specific carbohydrate interactionsTan, M.; Moore, S.; Tabor, R.; Fegan, N.; Rahman, S.; Dykes, Gary (2016)Background: Processing of fresh produce exposes cut surfaces of plant cell walls that then become vulnerable to human foodborne pathogen attachment and contamination, particularly by Salmonella enterica. Plant cell walls ...
Nguyen, V.; Turner, M.; Dykes, Gary (2011)This work aimed to investigate the influence of physicochemical properties and prior mode of growth (planktonic or sessile culture) on attachment of 13 Campylobacter jejuni strains and 5 Campylobacter coli strains isolated ...
Role of capsular polysaccharides and lipooligosaccharides in campylobacter surface properties, autoagglutination, and attachment to abiotic surfacesNguyen, V.; Barlow, R.; Fegan, N.; Turner, M.; Dykes, Gary (2013)The role of capsular polysaccharides and lipooligosaccharides in cell surface hydrophobicity, surface charge, autoagglutination (AAG), and attachment to abiotic surfaces of three strains of Campylobacter jejuni and one ...