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    Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa

    Access Status
    Open access
    Authors
    Tian, Z.
    Fargier, E.
    Mac Aogáin, M.
    Adams, C.
    Wang, Y.
    O'Gara, Fergal
    Date
    2009
    Type
    Journal Article
    
    Metadata
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    Citation
    Tian, Z. and Fargier, E. and Mac Aogáin, M. and Adams, C. and Wang, Y. and O'Gara, F. 2009. Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa. Nucleic Acids Research. 37 (22): pp. 7546-7559.
    Source Title
    Nucleic Acids Research
    DOI
    10.1093/nar/gkp828
    ISSN
    0305-1048
    Remarks

    This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by-nc/2.5/uk/

    URI
    http://hdl.handle.net/20.500.11937/31171
    Collection
    • Curtin Research Publications
    Abstract

    The LysR-family regulator MexT modulates the expression of the MexEF-OprN efflux system in the human pathogen Pseudomonas aeruginosa. Recently, we demonstrated that MexT regulates certain virulence phenotypes, including the type-three secretion system and early attachment independent of its role in regulating MexEF-OprN. In this study, transcriptome profiling was utilized to investigate the global nature of MexT regulation in P. aeruginosa PAO1 and an isogenic mexEF mutant. Twelve genes of unknown function were highly induced by overexpressing MexT independent of MexEF-OprN. A well-conserved DNA motif was identified in the upstream regulatory region of nine of these genes and upstream of mexE. Reporter fusion analysis demonstrated that the expression of the genes was significantly induced by MexT in P. aeruginosa and a heterogenous Escherichia coli strain and that the conserved sequence was required for this induction. The conserved DNA motif was further characterized as the MexT binding site by site-directed mutagenesis and electrophoretic mobility shift assays. Genes containing this conserved regulatory sequence were identified across other Pseudomonas species, and their expression was activated by MexT. Thus, a novel regulon directly modulated by MexT, that includes but is independent of mexEF-oprN, has been identified.

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