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    Microsatellite primers for the rare sedge Lepidosperma bungalbin (Cyperaceae)

    242020_242020.pdf (511.1Kb)
    Access Status
    Open access
    Authors
    Nevill, Paul
    Wardell-Johnson, G.
    Date
    2016
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Nevill, P. and Wardell-Johnson, G. 2016. Microsatellite primers for the rare sedge Lepidosperma bungalbin (Cyperaceae). Applications in Plant Sciences. 4 (11): 1600083.
    Source Title
    Appl Plant Sci
    DOI
    10.3732/apps.1600083
    ISSN
    2168-0450
    School
    Department of Environment and Agriculture
    Funding and Sponsorship
    http://purl.org/au-research/grants/arc/IC150100041
    Remarks

    This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by-nc-sa/4.0/

    URI
    http://hdl.handle.net/20.500.11937/31390
    Collection
    • Curtin Research Publications
    Abstract

    Premise of the study: Microsatellite markers were developed for the rare sedge Lepidosperma bungalbin (Cyperaceae) to assess genetic variation and its spatial structuring. Methods and Results: We conducted shotgun sequencing on an Illumina MiSeq and produced 6,215,872 sequence reads. The QDD pipeline was used to design 60 primer pairs that were screened using PCR. We developed 17 loci, of which 12 loci were identified that were polymorphic, amplified reliably, and could be consistently scored. We then screened these loci for variation in individuals from three populations. The number of alleles observed for these 12 loci across the three populations ranged from nine to 19 and expected heterozygosity ranged from 0.41 to 0.89. Conclusions: These markers will enable the quantification of the potential impact of mining on genetic variation within L. bungalbin and establish a baseline for future management of genetic variation of the rare sedge.

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