Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect

    Access Status
    Open access via publisher
    Authors
    Lareu, Ricky R.
    Subramhanya, H.
    Peng, Y.
    Benny, P.
    Chen, C.
    Wang, Z.
    Rajagopalan, R.
    Raghunath, M.
    Date
    2007
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Lareu, Ricky R. and Subramhanya, Karthik Harve and Peng, Yanxian and Benny, Paula and Chen, Clarice and Wang, Zhibo and Rajagopalan, Raj and Raghunath, Michael. 2007. Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect. FEBS Letters. 581 (14): pp. 2709-2714.
    Source Title
    FEBS Letters
    DOI
    10.1016/j.febslet.2007.05.020
    ISSN
    0014-5793
    URI
    http://hdl.handle.net/20.500.11937/31588
    Collection
    • Curtin Research Publications
    Abstract

    The excluded volume effect (EVE) rules all life processes. It is created by macromolecules that occupy a given volume thereby confining other molecules to the remaining space with large consequences on reaction kinetics and molecular assembly. Implementing EVE in fibroblast culture accelerated conversion of procollagen to collagen by procollagen C-proteinase (PCP/BMP-1) and proteolytic modification of its allosteric regulator, PCOLCE1. This led to a 20–30- and 3–6-fold increased collagen deposition in two- and three-dimensional cultures, respectively, and creation of crosslinked collagen footprints beneath cells. Important parameters correlating with accelerated deposition were hydrodynamic radius of macromolecules and their negative charge density.

    Related items

    Showing items related by title, author, creator and subject.

    • The Scar-in-a-Jar: studying potential anti?brotic compounds from the epigenetic to extracellular level in a single well
      Chen, C.; Peng, Y.; Wang, Z.; Fish, P.; Kaar, J.; Koepsel, R.; Russell, A.; Lareu, Ricardo; Raghunath, M. (2009)
      Background and purpose: Fibrosis, a pathological accumulation of collagen in tissues, represents a major global disease burden. Effective characterization of potential antifibrotic drugs has been constrained by poor ...
    • A histological and micro-CT investigation in to the effect of NGF and EGF on the periodontal, alveolar bone, root and pulpal healing of replanted molars in a rat model - a pilot study
      Furfaro, F.; Ang, E.; Lareu, Ricky; Murray, Kevin; Goonewardene, M. (2014)
      Background: This study aims to investigate, utilising micro-computed tomography (micro-CT) and histology, whether the topical application of nerve growth factor (NGF) and/or epidermal growth factor (EGF) can enhance ...
    • Synthesis and evaluation of porous composite hydrogels for tissue engineering applications
      Li, Chao (2012)
      The purpose of this dissertation was to synthesize and evaluate porous poly(2- hydroxyethyl methacrylate) (PHEMA) and PHEMA composite hydrogels containing various concentrations of titanium dioxide (TiO2) nanoparticles, ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.