Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Local control of phosphatidylinositol 4-phosphate signaling in the Golgi apparatus by Vps74 and Sac1 phosphoinositide phosphatase

    Access Status
    Open access via publisher
    Authors
    Wood, C.
    Hung, C.
    Huoh, Y.
    Mousley, Carl
    Stefan, C.
    Bankaitis, V.
    Ferguson, K.
    Burd, C.
    Date
    2012
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Wood, C. and Hung, C. and Huoh, Y. and Mousley, C. and Stefan, C. and Bankaitis, V. and Ferguson, K. et al. 2012. Local control of phosphatidylinositol 4-phosphate signaling in the Golgi apparatus by Vps74 and Sac1 phosphoinositide phosphatase. Molecular Biology of the Cell. 23 (13): pp. 2527-2536.
    Source Title
    Molecular Biology of the Cell
    DOI
    10.1091/mbc.E12-01-0077
    ISSN
    1059-1524
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/31780
    Collection
    • Curtin Research Publications
    Abstract

    In the Golgi apparatus, lipid homeostasis pathways are coordinated with the biogenesis of cargo transport vesicles by phosphatidylinositol 4-kinases (PI4Ks) that produce phosphatidylinositol 4-phosphate (PtdIns4P), a signaling molecule that is recognized by downstream effector proteins. Quantitative analysis of the intra-Golgi distribution of a PtdIns4P reporter protein confirms that PtdIns4P is enriched on the trans-Golgi cisterna, but surprisingly, Vps74 (the orthologue of human GOLPH3), a PI4K effector required to maintain residence of a subset of Golgi proteins, is distributed with the opposite polarity, being most abundant on cis and medial cisternae. Vps74 binds directly to the catalytic domain of Sac1 (KD = 3.8 µM), the major PtdIns4P phosphatase in the cell, and PtdIns4P is elevated on medial Golgi cisternae in cells lacking Vps74 or Sac1, suggesting that Vps74 is a sensor of PtdIns4P level on medial Golgi cisternae that directs Sac1-mediated dephosphosphorylation of this pool of PtdIns4P. Consistent with the established role of Sac1 in the regulation of sphingolipid biosynthesis, complex sphingolipid homeostasis is perturbed in vps74? cells. Mutant cells lacking complex sphingolipid biosynthetic enzymes fail to properly maintain residence of a medial Golgi enzyme, and cells lacking Vps74 depend critically on complex sphingolipid biosynthesis for growth. The results establish additive roles of Vps74-mediated and sphingolipid- dependent sorting of Golgi residents. © 2012 Wood et al.

    Related items

    Showing items related by title, author, creator and subject.

    • Functional Anatomy of Phospholipid Binding and Regulation of Phosphoinositide Homeostasis by Proteins of the Sec14 Superfamily
      Schaaf, G.; Ortlund, E.; Tyeryar, K.; Mousley, Carl; Ile, K.; Garrett, T.; Ren, J.; Woolls, M.; Raetz, C.; Redinbo, M.; Bankaitis, V. (2008)
      Sec14, the major yeast phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer protein, regulates essential interfaces between lipid metabolism and membrane trafficking from the trans-Golgi network (TGN). How ...
    • Trans-Golgi network and endosome dynamics connect ceramide homeostasis with regulation of the unfolded protein response and TOR signaling in yeast
      Mousley, Carl; Tyeryar, K.; Ile, K.; Schaaf, G.; Brost, R.; Boone, C.; Guan, X.; Wenk, M.; Bankaitis, V. (2008)
      Synthetic genetic array analyses identify powerful genetic interactions between a thermosensitive allele (sec14-1 ts)ofthe structural gene for the major yeast phosphatidylinositol transfer protein (SEC14) and a structural ...
    • PI3K Class II [alpha] Controls Spatially Restricted Endosomal PtdIns3P and Rab11 Activation to Promote Primary Cilium Function
      Franco, I.; Gulluni, F.; Campa, C.; Costa, C.; Margaria, J.; Ciraolo, E.; Martini, M.; Monteyne, D.; De Luca, E.; Germena, G.; Posor, Y.; Maffucci, T.; Marengo, S.; Haucke, V.; Falasca, Marco; Perez-Morga, D.; Boletta, A.; Merlo, G.; Hirsch, E. (2014)
      Multiple phosphatidylinositol (PtdIns) 3-kinases (PI3Ks) can produce PtdIns3P to control endocytic trafficking, but whether enzyme specialization occurs in defined subcellular locations is unclear. Here, we report that ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.