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    Adsorption and Unfolding of Lysozyme at a Polarized Aqueous-Organic Liquid Interface

    Access Status
    Fulltext not available
    Authors
    Arooj, M.
    Gandhi, N.
    Kreck, C.
    Arrigan, Damien
    Mancera, R.
    Date
    2016
    Type
    Journal Article
    
    Metadata
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    Citation
    Arooj, M. and Gandhi, N. and Kreck, C. and Arrigan, D. and Mancera, R. 2016. Adsorption and Unfolding of Lysozyme at a Polarized Aqueous-Organic Liquid Interface. Journal of Physical Chemistry B. 120 (12): pp. 3100-3112.
    Source Title
    Journal of Physical Chemistry B
    DOI
    10.1021/acs.jpcb.6b00536
    ISSN
    1520-6106
    School
    Nanochemistry Research Institute
    URI
    http://hdl.handle.net/20.500.11937/31988
    Collection
    • Curtin Research Publications
    Abstract

    The adsorption of proteins at the interface between two immiscible electrolyte solutions has been found to be key to their bioelectroactivity at such interfaces. Combined with interfacial complexation of organic phase anions by cationic proteins, this adsorption process may be exploited to achieve nanomolar protein detection. In this study, replica exchange molecular dynamics simulations have been performed to elucidate for the first time the molecular mechanism of adsorption and subsequent unfolding of hen egg white lysozyme at low pH at a polarized 1,2-dichloroethane/water interface. The unfolding of lysozyme was observed to occur as soon as it reaches the organic-aqueous interface, which resulted in a number of distinct orientations at the interface. In all cases, lysozyme interacted with the organic phase through regions rich in nonpolar amino acids, such that the side chains are directed toward the organic phase, whereas charged and polar residues were oriented toward the aqueous phase. By contrast, as expected, lysozyme in neat water at low pH does not exhibit significant structural changes. These findings demonstrate the key influence of the organic phase upon adsorption of lysozyme under the influence of an electric field, which results in the unfolding of its structure. (Figure Presented).

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