Short-term effect of low-intensity pulsed ultrasound on an ex-vivo 3-D tooth culture
dc.contributor.author | Al-Daghreer, S. | |
dc.contributor.author | Doschak, Michael | |
dc.contributor.author | Sloan, A. | |
dc.contributor.author | Major, P. | |
dc.contributor.author | Heo, G. | |
dc.contributor.author | Scurtescu, C. | |
dc.contributor.author | Tsui, Y. | |
dc.contributor.author | El-Bialy, T. | |
dc.date.accessioned | 2017-01-30T13:32:32Z | |
dc.date.available | 2017-01-30T13:32:32Z | |
dc.date.created | 2013-11-19T20:00:38Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Al-Daghreer, Saleh and Doschak, Michael and Sloan, Alastair J. and Major, Paul W. and Heo, Giseon and Scurtescu, Cristian and Tsui, Ying Y. and El-Bialy, Tarek. 2013. Short-term effect of low-intensity pulsed ultrasound on an ex-vivo 3-D tooth culture. Ultrasound in Medicine and Biology. 39 (6): pp. 1066-1074. | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/32709 | |
dc.identifier.doi | 10.1016/j.ultrasmedbio.2012.12.005 | |
dc.description.abstract |
We investigated the short-term effect of LIPUS on human dentin-pulp complex in vitro. We collected sixty-three premolars from patients who needed the extraction. The premolars were sectioned transversely into 600-µm-thick slices, and then divided into five groups according to LIPUS application time (control, 5, 10, 15 and 20 min). LIPUS transducer produced an incident intensity of 30 mW/cm2. After 24 h, tissue was harvested for histomorphometrical analysis and RT-PCR (Genes of interest: Collagen I, DMP1, DSPP, TGF ß1, RANKL and OPG). Histomorphometric analysis showed no significant difference among the five groups in the odontoblast count and predentin thickness. RT-PCR demonstrated no expression of TGF ß1, low amounts of DSPP, a twofold increase in collagen I expression in the 5- and 10-minute LIPUS groups and a threefold increase in DMP1 expression in the 10-minute LIPUS group. LIPUS application was stimulatory to the dentin-pulp complex in vitro and increased the expression of collagen I and DMP1. | |
dc.publisher | Elsevier Inc. | |
dc.subject | DMP1 | |
dc.subject | odontoblast | |
dc.subject | LIPUS | |
dc.subject | predentin | |
dc.subject | dentin-pulp complex | |
dc.title | Short-term effect of low-intensity pulsed ultrasound on an ex-vivo 3-D tooth culture | |
dc.type | Journal Article | |
dcterms.source.volume | 39 | |
dcterms.source.number | 6 | |
dcterms.source.startPage | 1066 | |
dcterms.source.endPage | 1074 | |
dcterms.source.issn | 0301-5629 | |
dcterms.source.title | Ultrasound in Medicine and Biology | |
curtin.department | ||
curtin.accessStatus | Fulltext not available |