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dc.contributor.authorTunlid, A.
dc.contributor.authorAhman, J.
dc.contributor.authorOliver, Richard
dc.identifier.citationTUNLID A, AHMAN J & OLIVER RP (1999) Transformation of the nematode-trapping fungus Arthobotrys oligospora. FEMS Microbiology Letters 173 111-116

The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaCl2 treatment. Transformation frequencies varied between 1–6 transformants per μg DNA. Seven out of 13 integration events analyzed from transformants were single copy integrations, whereas the remaining were multiple and more complex integrations. The addition of restriction enzymes during transformations increased the frequency of single copy integrations. Co-transformation, using the E. coli uidA gene encoding the β-glucuronidase reporter gene under the control of an Aspergillus nidulans promoter, occurred at frequencies of up to 63%.

dc.titleTransformation of the nematode-trapping fungus Arthrobotrys oligospora
dc.typeJournal Article

A copy of this item may be available from Professor Richard Oliver



curtin.accessStatusFulltext not available
curtin.facultyDepartment of Environmental & Agriculture
curtin.facultySchool of Agriculture and Environment
curtin.facultyFaculty of Science and Engineering

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