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dc.contributor.authorElnashar, Magdy
dc.contributor.authorKahil, T.
dc.date.accessioned2017-01-30T13:33:03Z
dc.date.available2017-01-30T13:33:03Z
dc.date.created2015-10-29T04:10:08Z
dc.date.issued2014
dc.date.submitted2015-10-29
dc.identifier.citationElnashar, M. and Kahil, T. 2014. Biopolymeric formulations for biocatalysis and biomedical applications. BioMed Research International. 2014 (Article ID 418097): pp. 1-10.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/32783
dc.identifier.doi10.1155/2014/418097
dc.description.abstract

Three gel disks formulations prepared using chitosan (Chito) or polyethylenimine (PEI) followed by glutaraldehyde were prepared for biocatalysis and biomedical applications. The carriers have been used to immobilize lactase covalently and it was evaluated in terms of enzyme loading capacity and enzyme kinetics (km and Vmax). The Km of the Chito formulation was almost half that of the PEI formulations, which is favored in industries. On the other hand, the gel disks were evaluated in terms of their swelling kinetics and the gels' morphology using SEM. The mechanism of the three gels' swelling was also studied and it was found to be non-Fickian, where the mechanism of transport depends on both the diffusion and polymer relaxation, which are controlling the overall rate of water uptake. The Chito formulation was 2-5 folds and PEI formulations were 33-62 folds in terms of the swelling rate constant and the diffusion rate, respectively. These results were highly supported by the SEM. This study will help scientists to design the right polymer network for enzymes immobilization as well as control the surface area and the swelling power of the polymers for different applications such as drug delivery systems and tissue engineering.

dc.publisherHindawi Publishing Corporation
dc.titleBiopolymeric formulations for biocatalysis and biomedical applications
dc.typeJournal Article
dcterms.dateSubmitted2015-10-29
dcterms.source.volume2014
dcterms.source.issn2314-6133
dcterms.source.titleBioMed Research International
curtin.digitool.pid233688
curtin.note

This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by/3.0/

curtin.pubStatusPublished
curtin.refereedTRUE
curtin.departmentSchool of Biomedical Sciences
curtin.identifier.scriptidPUB-VC-ORD-SA-24591
curtin.identifier.elementsidELEMENTS-100804
curtin.accessStatusOpen access


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