Heterologous expression of Septoria lycopersici tomatinase in Cladosporium fulvum: Effects on compatible and incompatible interactions with tomato seedlings
MetadataShow full item record
A copy of this item may be available from Professor Richard Oliver
The anti-fungal, steroidal, glycoalkaloid saponin, α-tomatine, is present in uninfected tomato plants in substantial concentrations, and may contribute to the protection of tomato plants against attack by phytopathogenic fungi. In general, successful fungal pathogens of tomato are more resistant to α-tomatine in vitro than fungi that do not infect this plant. For a number of tomato pathogens, this resistance has been associated with the ability to detoxify α-tomatine through the action of enzymes known as tomatinases. In contrast, the biotrophic tomato pathogen Cladosporium fulvum is sensitive to α-tomatine and is unable to detoxify this saponin. This paper describes the effects of heterologous expression of the cDNA encoding tomatinase from the necrotroph Septoria lycopersici in two different physiological races of C. fulvum. Tomatinase-producing C. fulvum transformants showed increased sporulation on cotyledons of susceptible tomato lines. They also caused more extensive infection of seedlings of resistant tomato lines. Thus, α-tomatine may contribute to the ability of tomato to restrict the growth of C. fulvum in both compatible and incompatible interactions.
Showing items related by title, author, creator and subject.
Evidence that γ-aminobutyric acid is a major nitrogen source during Cladosporium fulvum infection of tomatoSolomon, P.; Oliver, Richard (2002)The growth of the biotrophic pathogen Cladosporium fulvum within the tomato (Lycopersicon esculentum Mill.) leaf is restricted to the intercellular space. Previous studies from this laboratory have demonstrated that ...
Solomon, P.; Oliver, Richard (2001)To address the problem of the nutritional requirements of phyto-pathogenic fungi growing in planta, the environment for the intercellular biotrophic pathogen, Cladosporium fulvum Cooke, of tomato (Lycopersicon esculentum ...
Investigating the role of polyols in Cladosporium fulvum during growth under hyperosmotic stress and in plantaClark, A.; Blissett, K.; Oliver, Richard (2003)The role of the large amounts of polyols accumulated by the fungal tomato pathogen, Cladosporium fulvum (syn. Fulvia fulva, Cooke) both in planta and in axenic cultures has been examined. Arabinitol and glycerol accumulated ...