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    Integrated microspectrometer for fluorescence based analysis in a microfluidic format

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    Fulltext not available
    Authors
    Hu, Z.
    Glidle, A.
    Ironside, Charlie
    Sorel, M.
    Strain, M.
    Cooper, J.
    Yin, H.
    Date
    2012
    Type
    Journal Article
    
    Metadata
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    Citation
    Hu, Z. and Glidle, A. and Ironside, C. and Sorel, M. and Strain, M. and Cooper, J. and Yin, H. 2012. Integrated microspectrometer for fluorescence based analysis in a microfluidic format. Lab on a Chip - Miniaturisation for Chemistry and Biology. 12 (16): pp. 2850-2857.
    Source Title
    Lab on a Chip - Miniaturisation for Chemistry and Biology
    DOI
    10.1039/c2lc40169j
    ISSN
    1473-0197
    School
    Department of Physics and Astronomy
    URI
    http://hdl.handle.net/20.500.11937/33054
    Collection
    • Curtin Research Publications
    Abstract

    We have demonstrated a monolithic integrated arrayed waveguide grating (AWG) microspectrometer microfluidic platform capable of fluorescence spectroscopic analysis. The microspectrometer in this proof of concept study has a small (1 cm × 1 cm) footprint and 8 output channels centred on different wavelengths. We show that the signals from the output channels detected on a camera chip can be used to recreate the complete fluorescence spectrum of an analyte. By making fluorescence measurements of (i) mixed quantum dot solutions, (ii) an organic fluorophore (Cy5) and (iii) the propidium iodide (PI)-DNA assay, we illustrate the unique advantages of the AWG platform for simultaneous, quantitative multiplex detection and its capability to detect small spectroscopic shifts. Although the current system is designed for fluorescence spectroscopic analysis, in principle, it can be implemented for other types of analysis, such as Raman spectroscopy. Fabricated using established semiconductor industry methods, this miniaturised platform holds great potential to create a handheld, low cost biosensor with versatile detection capability. © The Royal Society of Chemistry 2012.

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