Integrated microspectrometer for fluorescence based analysis in a microfluidic format
MetadataShow full item record
We have demonstrated a monolithic integrated arrayed waveguide grating (AWG) microspectrometer microfluidic platform capable of fluorescence spectroscopic analysis. The microspectrometer in this proof of concept study has a small (1 cm × 1 cm) footprint and 8 output channels centred on different wavelengths. We show that the signals from the output channels detected on a camera chip can be used to recreate the complete fluorescence spectrum of an analyte. By making fluorescence measurements of (i) mixed quantum dot solutions, (ii) an organic fluorophore (Cy5) and (iii) the propidium iodide (PI)-DNA assay, we illustrate the unique advantages of the AWG platform for simultaneous, quantitative multiplex detection and its capability to detect small spectroscopic shifts. Although the current system is designed for fluorescence spectroscopic analysis, in principle, it can be implemented for other types of analysis, such as Raman spectroscopy. Fabricated using established semiconductor industry methods, this miniaturised platform holds great potential to create a handheld, low cost biosensor with versatile detection capability. © The Royal Society of Chemistry 2012.
Showing items related by title, author, creator and subject.
Application of Solvent Influenced Fluorescence-quenching and Enhancement to Develop a Highly Sensitive HPLC Methodology for Analysis of Resveratrol-PEG ConjugatesBasavaraj, S; Benson, Heather; Brown, David; Chen, Yan (2013)Quantification of pegylated drugs in biological matrices is often complex and challenging. This paper describes a sensitive HPLC method with fluorescence detection for the analysis of pegylated resveratrol, resveratrol ...
Size exclusion chromatography as a tool for natural organic matter characterisation in drinking water treatmentAllpike, Bradley (2008)Natural organic matter (NOM), ubiquitous in natural water sources, is generated by biogeochemical processes in both the water body and in the surrounding watershed, as well as from the contribution of organic compounds ...
Zhang, Z.; Greenwood, Paul; Zhang, Q.; Rao, D.; Shi, W. (2012)The hydrocarbon composition of co-occurring blue and yellow fluorescing fluid inclusions (FIs) in Ordovician reservoirs of the Tarim Basin, NW China have been separately analyzed by laser microprobe gas chromatography–mass ...