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    A Simple Microfluidic Chip Design for Fundamental Bioseparation

    Access Status
    Open access via publisher
    Authors
    Chan, A.
    Danquah, Michael
    Agyei, D.
    Hartley, P.
    Zhu, Y.
    Date
    2014
    Type
    Journal Article
    
    Metadata
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    Citation
    Chan, A. and Danquah, M. and Agyei, D. and Hartley, P. and Zhu, Y. 2014. A Simple Microfluidic Chip Design for Fundamental Bioseparation. Journal of Analytical Methods in Chemistry. Article ID 175457 (6 pp.).
    Source Title
    Journal of Analytical Methods in Chemistry
    DOI
    10.1155/2014/175457
    ISSN
    2090-8865
    School
    Department of Chemical Engineering
    URI
    http://hdl.handle.net/20.500.11937/33262
    Collection
    • Curtin Research Publications
    Abstract

    A microchip pressure-driven liquid chromatographic system with a packed column has been designed and fabricated by using poly(dimethylsiloxane) (PDMS). The liquid chromatographic column was packed with mesoporous silica beads of Ia3d space group. Separation of dyes and biopolymers was carried out to verify the performance of the chip. A mixture of dyes (fluoresceinand rhodamine B) and a biopolymer mixture (10 kDa Dextran and 66 kDa BSA) were separated and the fluorescence technique was employed to detect the movement of the molecules. Fluorescein molecule was a nonretained species and rhodamine B was attached onto silica surface when dye mixture in deionized water was injected into the microchannel. The retention times for dextran molecule and BSA molecule in biopolymer separation experiment were 45 s and 120 s, respectively. Retention factor was estimated to be 3.3 for dextran and 10.4 for BSA. The selectivity was 3.2 and resolution was 10.7. Good separation of dyes and biopolymers was achieved and the chip design was verified.

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