A Simple Microfluidic Chip Design for Fundamental Bioseparation
MetadataShow full item record
A microchip pressure-driven liquid chromatographic system with a packed column has been designed and fabricated by using poly(dimethylsiloxane) (PDMS). The liquid chromatographic column was packed with mesoporous silica beads of Ia3d space group. Separation of dyes and biopolymers was carried out to verify the performance of the chip. A mixture of dyes (fluoresceinand rhodamine B) and a biopolymer mixture (10 kDa Dextran and 66 kDa BSA) were separated and the fluorescence technique was employed to detect the movement of the molecules. Fluorescein molecule was a nonretained species and rhodamine B was attached onto silica surface when dye mixture in deionized water was injected into the microchannel. The retention times for dextran molecule and BSA molecule in biopolymer separation experiment were 45 s and 120 s, respectively. Retention factor was estimated to be 3.3 for dextran and 10.4 for BSA. The selectivity was 3.2 and resolution was 10.7. Good separation of dyes and biopolymers was achieved and the chip design was verified.
Showing items related by title, author, creator and subject.
Contribution of different effluent organic matter fractions to membrane fouling in ultrafiltration of treated domestic wastewaterZheng, X.; Croue, Jean-Philippe (2012)In the present work, effluent organic matter (EfOM) in treated domestic wastewater was separated into hydrophobic neutrals, colloids, hydrophobic acids, transphilic acids and neutrals and hydrophilic compounds. Their ...
The culture of Chlorella vulgaris in a recycled supernatant: Effects on biomass production and medium qualityHadj-Romdhane, F.; Zheng, X.; Jaouen, P.; Pruvost, J.; Grizeau, D.; Croué, Jean-Philippe; Bourseau, P. (2013)Reusing supernatant of microalgae culture medium can have inhibitory or toxic effects on the biomass production because of the release of organic metabolites by cells in the culture medium during their growth. This work ...
Biophysical characterization of layer-by-layer synthesis of aptamer-drug microparticles for enhanced cell targetingTan, K.; Danquah, M.; Sidhu, Amandeep; Lau, Shiew Wei; Ongkudon, C. (2017)© 2017 American Institute of Chemical Engineers. Targeted delivery of drug molecules to specific cells in mammalian systems demonstrates a great potential to enhance the efficacy of current pharmaceutical therapies. ...