Detection and discovery of novel arboviruses in Western Australia
MetadataShow full item record
Viruses from several recognized arthropod-borne virus families are regularly isolated in Australia. Many of these are important human and animal pathogens, and the emergence of novel or exotic arboviral pathogens pose additional threats. In Western Australia, arbovirus activity is monitored by the Arbovirus Surveillance and Research Laboratory. Viruses are isolated in cell culture from pools of mosquitoes, and their identification is then performed using a panel of monoclonal antibodies raised against medically important viruses belonging to the Alphavirus and Flavivirus genera in an enzyme immunoassay. A collection of arbovirus cultures that had evaded serological identification were further screened using molecular methods. A series of RT-PCR assays using primer sets targeting alphaviruses, flaviviruses and orbiviruses enabled identification of many of these cultures. The identity of the remaining isolates was subsequently determined using unbiased high-throughput sequencing. Novel members of the Rhabdoviridae (Oak Vale-like virus) and Reoviridae (Stretch Lagoon orbivirus) were identified. Viruses belonging to the Mapputta virus group of the Orthobunyaviridae family were also identified, as well as a single isolate of a novel reassortant virus, whose genome consists of a bunyamwera virus-like L segment and Batai virus-like M and S segments. The identification and characterisation of these viruses has informed subsequent molecular screening activities. These findings emphasise the importance of employing combinations of laboratory-based diagnostic and detection methods for comprehensive arbovirus surveillance.
Showing items related by title, author, creator and subject.
Analysis of arbovirus isolates from Australia identifies novel bunyaviruses including a mapputta group virus from Western Australia that links gan gan and maprik virusesBriese, T.; Williams, D.; Kapoor, V.; Diviney, S.; Certoma, A.; Wang, J.; Johansen, C.; Chowdhary, R.; Mackenzie, John; Lipkin, W. (2016)The Mapputta group comprises antigenically related viruses indigenous to Australia and Papua New Guinea that are included in the family Bunyaviridae but not currently assigned to a specific genus. We determined and analyzed ...
Ong, J.; Li, H.; Sivasithamparam, K.; Dixon, Kingsley; Jones, M.; Wylie, S. (2018)© 2017 Elsevier B.V. Terrestrial orchids represent a symbiotic union between plants and mycorrhizal fungi. This study describes the occurrence and nature of viruses associated with one population of wild Pterostylis ...
Novel reassortant H9N2 viruses in pigeons and evidence for antigenic diversity of H9N2 viruses isolated from quails in EgyptKandeil, A.; El-Shesheny, R.; Maatouq, A.; Moatasim, Y.; Cai, Z.; McKenzie, P.; Webby, R.; Kayali, G.; Ali, Mohammed (2017)© 2017 The Authors. The endemicity of avian influenza viruses (AIVs) among Egyptian poultry represents a public health risk. Co-circulation of low pathogenic AIV H9N2 subtype with highly pathogenic AIV H5N1 subtype in ...