Electrochemiluminescence determination of thioridazine based on carbon nanofiber paste electrode

Abstract

A carbon nanofiber paste electrode (CFPE) was employed for fabrication of electrochemilumunescence (ECL) sensor. The performance of CFPE was characterized by cyclic voltammetry. The electrochemical and ECL behaviors of Ru(bpy)(3)(2+) and thioridazine (TR) were investigated. The electrode showed an excellent electrochemical activity and ECL response. Based on the fact that the light emission of the ECL sensor could be enhanced by TR dramatically, a new method has been established for the determination of TR. The effects of pH value and concentration of buffer, Ru(bpy)(3)(2+) were studied respectively. Under the optimum experimental conditions, the ECL sensor exhibited excellent linear response to TR ranging from 2 to 40 mu mol/L (R=0. 998) and a low detection limit of 0. 5 mu mol/L (S/N=3). The relative standard deviation was 1.1% for 15 mu mol/L TR (n=11). The established method was successfully applied for the determination of TR in urine sample with satisfactory results, and the recovery was between 98. 7% and 105. 4%.