Improving heterologous protein secretion at aerobic conditions by activating hypoxia-induced genes in Saccharomyces cerevisiae
MetadataShow full item record
Oxygen is important for normal aerobic metabolism, as well as for protein production where it is needed for oxidative protein folding. However, several studies have reported that anaerobic conditions seem to be more favorable in terms of recombinant protein production. We were interested in increasing recombinant protein production under aerobic conditions so we focused on Rox1p regulation. Rox1p is a transcriptional regulator, which in oxidative conditions represses genes induced in hypoxia. We deleted ROX1 and studied the effects on the production of recombinant proteins in Saccharomyces cerevisiae. Intriguingly, we found a 100% increase in the recombinant fungal a-amylase yield, as well as productivity. Varied levels of improvements were also observed for the productions of the human insulin precursor and the yeast endogenous enzyme invertase. Based on the genome-wide transcriptional response, we specifically focused on the effect of UPC2 upregulation on protein production and suggested a possible mechanistic explanation.
Showing items related by title, author, creator and subject.
Expression and modulation of tissue factor and tissue factor pathway inhibitor in an endothelial cell based modelEllery, Paul E. R. (2008)Haemostasis is a complex physiological process involving cellular and plasma protein components that interact to keep the blood fluid under normal conditions and prevent blood loss after vessel injury by promoting clot ...
Chiong, T.; Acquah, C.; Lau, John; Khor, Ee; Danquah, M. (2016)© 2016 Elsevier Inc. All rights reserved. Microalgal bioprocesses have emerged as sustainable value-adding processes for the development of biochemicals and biomolecules for a wide range of applications to meet vital ...
Engineering the oxygen sensing regulation results in an enhanced recombinant human hemoglobin production by Saccharomyces cerevisiaeMartínez, J.; Liu, Lifang; Petranovic, D.; Nielsen, J. (2015)Efficient production of appropriate oxygen carriers for transfusions (blood substitutes or artificial blood) has been pursued for many decades, and to date several strategies have been used, from synthetic polymers to ...