Synergistic Role of Bacterial Urease and Carbonic Anhydrase in Carbonate Mineralization
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Authors
Dhami, N.
Reddy, M.
Mukherjee, Abhijit
Date
2014Type
Journal Article
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Dhami, Navdeep Kaur and Reddy, M.S. and Mukherjee, Abhijit. 2014. Synergistic Role of Bacterial Urease and Carbonic Anhydrase in Carbonate Mineralization. Applied Biochemistry and Biotechnology. 172 (5): pp. 2552-2561.
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Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
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Abstract
The investigation on the synergistic role of urease (UA) and carbonic anhydrase (CA) in biomineralization of calcium carbonate in Bacillus megaterium suggested that the precipitation of CaCO3 is significantly faster in bacterial culture than in crude enzyme solutions. Calcite precipitation is significantly reduced when both the enzymes are inhibited in comparison with those of the individual enzyme inhibitions indicating that both UA and CA are crucial for efficient mineralization. Carbonic anhydrase plays a role in hydrating carbon dioxide to bicarbonate, while UA aids in maintaining the alkaline pH that promotes calcification process.
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