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    Synergistic Role of Bacterial Urease and Carbonic Anhydrase in Carbonate Mineralization

    Access Status
    Fulltext not available
    Authors
    Dhami, N.
    Reddy, M.
    Mukherjee, Abhijit
    Date
    2014
    Type
    Journal Article
    
    Metadata
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    Citation
    Dhami, Navdeep Kaur and Reddy, M.S. and Mukherjee, Abhijit. 2014. Synergistic Role of Bacterial Urease and Carbonic Anhydrase in Carbonate Mineralization. Applied Biochemistry and Biotechnology. 172 (5): pp. 2552-2561.
    Source Title
    Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
    DOI
    10.1007/s12010-013-0694-0
    ISSN
    0273-2289
    URI
    http://hdl.handle.net/20.500.11937/33569
    Collection
    • Curtin Research Publications
    Abstract

    The investigation on the synergistic role of urease (UA) and carbonic anhydrase (CA) in biomineralization of calcium carbonate in Bacillus megaterium suggested that the precipitation of CaCO3 is significantly faster in bacterial culture than in crude enzyme solutions. Calcite precipitation is significantly reduced when both the enzymes are inhibited in comparison with those of the individual enzyme inhibitions indicating that both UA and CA are crucial for efficient mineralization. Carbonic anhydrase plays a role in hydrating carbon dioxide to bicarbonate, while UA aids in maintaining the alkaline pH that promotes calcification process.

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