A step towards long-wavelength protein crystallography: Subjecting protein crystals to a vacuum
dc.contributor.author | Panjikar, S. | |
dc.contributor.author | Thomsen, L. | |
dc.contributor.author | O'Donnell, Kane | |
dc.contributor.author | Riboldi-Tunnicliffe, A. | |
dc.date.accessioned | 2017-01-30T13:41:44Z | |
dc.date.available | 2017-01-30T13:41:44Z | |
dc.date.created | 2015-10-29T04:09:18Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Panjikar, S. and Thomsen, L. and O'Donnell, K. and Riboldi-Tunnicliffe, A. 2015. A step towards long-wavelength protein crystallography: Subjecting protein crystals to a vacuum. Journal of Applied Crystallography. 48: pp. 913-916. | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/34173 | |
dc.identifier.doi | 10.1107/S1600576715006147 | |
dc.description.abstract |
Using the UHV experimental endstation on the soft X-ray beamline at the Australian Synchrotron, lysozyme and proteinase K crystals have been exposed to a vacuum of 10-5 mbar, prior to flash-cooling in a bath of liquid nitrogen. Subsequent data collection on the MX2 beamline at the Australian Synchrotron demonstrated that, for lysozyme and proteinase K, it is possible to subject these mounted crystals to a vacuum pressure of 10-5 mbar without destroying the crystal lattice. Despite the lower data quality of the vacuum-pumped crystals compared with control crystals, it is demonstrated that the protein crystals can survive in a vacuum under suitable conditions. | |
dc.publisher | International Union of Crystallography | |
dc.title | A step towards long-wavelength protein crystallography: Subjecting protein crystals to a vacuum | |
dc.type | Journal Article | |
dcterms.source.volume | 48 | |
dcterms.source.startPage | 913 | |
dcterms.source.endPage | 916 | |
dcterms.source.issn | 0021-8898 | |
dcterms.source.title | Journal of Applied Crystallography | |
curtin.department | Department of Physics and Astronomy | |
curtin.accessStatus | Open access via publisher |
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