Encapsulation capacity of Artemia nauplii with customized probiotics for use in the cultivation ofwestern king prawns (Penaeus latisulcatus Kishinouye, 1896)

Abstract

The encapsulation capacity of Artemia nauplii with customized probiotics Pseudomonas synxantha and Pseudomonas aeruginosa for use in the cultivation of western king prawns (Penaeus latisulcatus) was investigated. Seven trials were conducted to investigate this encapsulation capacity in terms of Artemia survival and probiotic load in Artemia. Newly hatched Artemia nauplii at 250 nauplii mL1 were fed individual probiotics at 0, 103, 105 and 107 colony-forming units (CFU) per millilitre, and mixtures of these two probiotics (105 CFUmL1) at 30:70,50:50 or 70:30 v/v in a medium of ozonated water (OW), tryptone soya broth (TSB), and a mixture of these media. The appropriate medium for encapsulation of probiotics by Artemia nauplii was the mixture of OWand TSB at 75:25 v/v; whereas, the use of OWor TSB alone was not efective. Artemia nauplii most efectively encapsulated the customized probiotics at 105 CFUmL1. The results indicates that the encapsulation of Artemia nauplii is optimized by using a combination of P. synxantha and P. aeruginosa at 50:50 v/v in a media mixture of OW and TSB at 75: 25 v/v. Artemia should be harvested at 48 h when survival is still high (78%) and the probiotic load in Artemia is high (3 104 CFUnauplius1).