Predictive mutational bioinformatic analysis of variation in the skin and wool associated corneodesmosin (CDSN) gene in sheep
MetadataShow full item record
Corneodesmosin (CDSN) is an important component of the desmosome in the epidermal cornified stratum and inner root sheath of hair follicles. DNA from a sheep BAC clone previously identified by us to contain CDSN was PCR amplified using cattle-derived primers and the product sequenced. A region of 4579 bp containing CDSN was shown to contain two exons separated by one intron and spanning 3683 bp. The DNA encodes a predicted protein of 546 amino acids. Phylogenetic analysis shows that sheep CDSN falls within a clade containing cattle and other ruminant-like species. Comparison of sequences generated from 12 unrelated merino sheep and the International Sheep Genome Consortium (ISGC) data identified 58 single nucleotide polymorphisms (SNPs) within the 4579 bp region of which 16 are contained within coding sequences (1 in 80 bp). The SNPs identified in this study will add to the Major Histocompatibility Complex (MHC) SNP panel, which will allow extensive haplotyping of the sheep MHC in future studies.
Showing items related by title, author, creator and subject.
The effect of diet on the nutrition and production of merino ewes in the arid shrublands of Western AustraliaDaly, Fiona Frances Margaret (2009)For the Arid Shrublands of Western Australia (WA) knowledge is limited on what sheep eat and how nutritious their diets are. A study was undertaken on two stations near Yalgoo (28º18’S 116º38’E) in WA, from November 2005 ...
Siva Subramaniam, Nitthiya (2012)The major histocompatibility complex (MHC) is a chromosomal region associated with immune responsiveness in vertebrates. Over four decades many studies have demonstrated important associations between MHC loci and disease ...
Lee, Chee Yang (2009)The main aim of this project has been to provide insights into the genomic organization of the class II region of the ovine major histocompatibility complex (MHC), a chromosomal region containing genes that control adaptive ...