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dc.contributor.authorSchmerk, C.
dc.contributor.authorWelander, P.
dc.contributor.authorHamad, M.
dc.contributor.authorBain, K.
dc.contributor.authorBernards, M.
dc.contributor.authorSummons, Roger
dc.contributor.authorValvano, M.
dc.date.accessioned2017-01-30T13:48:55Z
dc.date.available2017-01-30T13:48:55Z
dc.date.created2016-02-28T19:30:32Z
dc.date.issued2015
dc.identifier.citationSchmerk, C. and Welander, P. and Hamad, M. and Bain, K. and Bernards, M. and Summons, R. and Valvano, M. 2015. Elucidation of the Burkholderia cenocepacia hopanoid biosynthesis pathway uncovers functions for conserved proteins in hopanoid-producing bacteria. Environmental Microbiology. 17 (3): pp. 735-750.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/35311
dc.identifier.doi10.1111/1462-2920.12509
dc.description.abstract

© 2014 Society for Applied Microbiology and John Wiley & Sons Ltd. Hopanoids are bacterial surrogates of eukaryotic membrane sterols and among earth's most abundant natural products. Their molecular fossils remain in sediments spanning more than a billion years. However, hopanoid metabolism and function are not fully understood. Burkholderia species are environmental opportunistic pathogens that produce hopanoids and also occupy diverse ecological niches. We investigated hopanoids biosynthesis in Burkholderia cenocepacia by deletion mutagenesis and structural characterization of the hopanoids produced by the mutants. The enzymes encoded by hpnH and hpnG were essential for production of all C35 extended hopanoids, including bacteriohopanetetrol (BHT), BHT glucosamine and BHT cyclitol ether. Deletion of hpnI resulted in BHT production, while ?hpnJ produced only BHT glucosamine. Thus, HpnI is required for BHT glucosamine production while HpnJ is responsible for its conversion to the cyclitol ether. The ?hpnH and ?hpnG mutants could not grow under any stress condition tested, whereas ?hpnI, ?hpnJ and ?hpnK displayed wild-type growth rates when exposed to detergent, but varying levels of sensitivity to low pH and polymyxin B. This study not only elucidates the biosynthetic pathway of hopanoids in B.cenocepacia, but also uncovers a biosynthetic role for the conserved proteins HpnI, HpnJ and HpnK in other hopanoid-producing bacteria.

dc.publisherBlackwell Publishing Ltd
dc.titleElucidation of the Burkholderia cenocepacia hopanoid biosynthesis pathway uncovers functions for conserved proteins in hopanoid-producing bacteria
dc.typeJournal Article
dcterms.source.volume17
dcterms.source.number3
dcterms.source.startPage735
dcterms.source.endPage750
dcterms.source.issn1462-2912
dcterms.source.titleEnvironmental Microbiology
curtin.accessStatusFulltext not available


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