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    Studies of the Rate Constant of L-DOPA Oxidation and Decarboxylation by HPLC

    Access Status
    Fulltext not available
    Authors
    Zhou, Y.
    Alany, R.
    Chuang, Victor
    Wen, J.
    Date
    2012
    Type
    Journal Article
    
    Metadata
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    Citation
    Zhou, Yong Zhi and Alany, Raid G. and Chuang, Victor and Wen, Jingyuan. 2012. Studies of the Rate Constant of L-DOPA Oxidation and Decarboxylation by HPLC. Chromatographia. 75 (11-12): pp. 597-606.
    Source Title
    Chromatographia
    DOI
    10.1007/s10337-012-2229-1
    ISSN
    00095893
    URI
    http://hdl.handle.net/20.500.11937/35846
    Collection
    • Curtin Research Publications
    Abstract

    The objective of current investigation was to study the degradation behavior of L-DOPA under different conditions by high performance liquid chromatography (HPLC), and to develop and validate a stability-indicating HPLC method. The developed RP-HPLC method was validated with respect to linearity, accuracy, precision and specificity. Oxidation was found to occur in alkaline and to some extent in thermal conditions, while the drug was stable when incubated at acidic conditions and under photolytic stress. The oxidation of L-DOPA was observed to follow first-order kinetics. The degradation rate constants and half-life were calculated. The cytotoxicity and enzymatic degradation of L-DOPA was examined using the human intestinal epithelial Caco-2 cells. The drug was rapidly decarboxylated by aromatic amino acid decarboxylase to dopamine. The conversion of L-DOPA to dopamine was dose - and time-dependent.

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