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    Molecular genetic and physical analysis of gas vesicles in buoyant enterobacteria

    238981_238981.pdf (588.9Kb)
    Access Status
    Open access
    Authors
    Tashiro, Y.
    Monson, R.
    Ramsay, Joshua
    Salmond, G.
    Date
    2016
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Tashiro, Y. and Monson, R. and Ramsay, J. and Salmond, G. 2016. Molecular genetic and physical analysis of gas vesicles in buoyant enterobacteria. Environmental Microbiology. 18 (4): pp. 1264-1276.
    Source Title
    Environmental Microbiology
    DOI
    10.1111/1462-2920.13203
    ISSN
    1462-2912
    School
    School of Biomedical Sciences
    Remarks

    This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    URI
    http://hdl.handle.net/20.500.11937/36437
    Collection
    • Curtin Research Publications
    Abstract

    © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd. Different modes of bacterial taxis play important roles in environmental adaptation, survival, colonization and dissemination of disease. One mode of taxis is flotation due to the production of gas vesicles. Gas vesicles are proteinaceous intracellular organelles, permeable only to gas, that enable flotation in aquatic niches. Gene clusters for gas vesicle biosynthesis are partially conserved in various archaea, cyanobacteria, and some proteobacteria, such as the enterobacterium, Serratia sp. ATCC 39006 (S39006). Here we present the first systematic analysis of the genes required to produce gas vesicles in S39006, identifying how this differs from the archaeon Halobacterium salinarum. We define 11 proteins essential for gas vesicle production. Mutation of gvpN or gvpV produced small bicone gas vesicles, suggesting that the cognate proteins are involved in the morphogenetic assembly pathway from bicones to mature cylindrical forms. Using volumetric compression, gas vesicles were shown to comprise 17% of S39006 cells, whereas in Escherichia coli heterologously expressing the gas vesicle cluster in a deregulated environment, gas vesicles can occupy around half of cellular volume. Gas vesicle production in S39006 and E.coli was exploited to calculate the instantaneous turgor pressure within cultured bacterial cells; the first time this has been performed in either strain.

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