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dc.contributor.authorPallebage-Gamarallage, Menuka
dc.contributor.authorGalloway, Susan
dc.contributor.authorTakechi, Ryusuke
dc.contributor.authorDhaliwal, Satvinder
dc.contributor.authorMamo, John
dc.date.accessioned2017-01-30T13:56:33Z
dc.date.available2017-01-30T13:56:33Z
dc.date.created2011-12-13T20:00:59Z
dc.date.issued2011
dc.identifier.citationPallebage-Gamarallage, Menuka and Galloway, Susan and Takechi, Ryusuke and Dhaliwal, Satvinder and Mamo, John. 2011. Probucol Suppresses Enterocytic Accumulation of Amyloid-β Induced by Saturated Fat and Cholesterol Feeding. Lipids. 47 (1): pp. 27-34.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/36581
dc.description.abstract

Amyloid-β (Aβ) is secreted from lipogenic organs such as intestine and liver as an apolipoprotein of nascent triacylglycerol rich lipoproteins. Chronically elevated plasma Aβ may compromise cerebrovascular integrity and exacerbate amyloidosis—a hallmark feature of Alzheimer’s disease (AD). Probucol is a hypocholesterolemic agent that reduces amyloid burden in transgenic amyloid mice, but the mechanisms for this effect are presently unclear. In this study, the effect of Probucol on intestinal lipoprotein-Aβ homeostasis was explored. Wild-type mice were fed a control low-fat diet and enterocytic Aβ was stimulated by high-fat (HF) diet enriched in 10% (w/w) saturated fat and 1% (w/w) cholesterol for the duration of 1 month. Mice treated with Probucol had the drug incorporated into the chow at 1% (w/w). Quantitative immunofluorescence was utilised to determine intestinal apolipoprotein B (apo B) and Aβ abundance. We found apo B in both the perinuclear region of the enterocytes and the lacteals in all groups. However, HF feeding and Probucol treatment increased secretion of apo B into the lacteals without any change in net villi abundance. On the other hand, HF-induced enterocytic perinuclear Aβ was significantly attenuated by Probucol. No significant changes in Aβ were observed within the lacteals. The findings of this study support the notion that Probucol suppresses dietary fat induced stimulation of Aβ biosynthesis and attenuate availability of apo B lipoprotein-Aβ for secretion.

dc.publisherSpringer
dc.titleProbucol Suppresses Enterocytic Accumulation of Amyloid-β Induced by Saturated Fat and Cholesterol Feeding
dc.typeJournal Article
dcterms.source.volume10
dcterms.source.startPage1
dcterms.source.endPage8
dcterms.source.issn00244201
dcterms.source.titleLipids
curtin.note

The final publication is available at link.springer.com

curtin.departmentSchool of Public Health
curtin.accessStatusOpen access


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