A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite
MetadataShow full item record
In this paper, an amperometric biosensor of hydrogen peroxide (H2O2) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration (Γ*) and apparent heterogeneous electron transfer rate constant (ks) were calculated to be (1.60 ± 0.17) × 10−10 mol cm−2 and 48.51 s−1, respectively. In addition, the Hb/Pluronic P123-NGP composite showed excellent bioelectrocatalytic activity toward the reduction of H2O2. The biosensor of H2O2 exhibited a linear response to H2O2 in the range of 10–150 μM and a detection limit of 8.24 μM (S/N = 3) was obtained. The apparent Michaelis–Menten constant (K app/m) was 45.35 μM. The resulting biosensor showed fast amperometric response, with very high sensitivity, reliability and effectiveness.
Showing items related by title, author, creator and subject.
Strategies to Achieve Control over the Surface Ratio of Two Different Components on Modified Electrodes Using Aryldiazonium SaltsJiang, C.; Alam, M.; Parker, S.; Darwish, Nadim; Gooding, J. (2016)Controlling the composition of an interface is very important in tuning the chemical and physical properties of a surface in many applications including biosensors, biomaterials, and chemical catalysis. Frequently, this ...
Pejcic, Bobby; De Marco, Roland; Parkinson, Gordon (2006)Global biosecurity threats such as the spread of emerging infectious diseases (i.e., avian influenza, SARS, Hendra, Nipah, etc.) and bioterrorism have generated significant interest in recent years. There is considerable ...
Chemical oxidative polymerization of conductive polyaniline-iron oxide composite as an electro-transducer for electrochemical sensing applicationsJawad, Zeinab (2016)This study explored the preparation of conductive polyaniline-iron oxide (PANI-Fe2O3) that served as the electrical signal transducer, to convert the electrochemical interactions between the biotinylated-goat-anti-mouse ...