Introducing a New Family of Biotinylated Ir(III)-Pyridyltriazole Lumophores: Synthesis, Photophysics, and Preliminary Study of Avidin-Binding Properties
MetadataShow full item record
Six new biotinylated reagents derived from monocationic pyridyl-1,2,3-triazole-based Ir(III) complexes of the general formula [(C∧N)2Ir(N∧N-spacer-X-CO-biotin)]+, where HC∧N is 2-phenylpyridine, N∧N is the neutral chelating (2-pyridyl)-1,2,3-triazole ligand, the term “spacer” refers to alkyl (−C11H22) or aromatic (p-phenyl- or 4,4′-biphenyl-) chains, and X is NH or O, have been prepared and characterized. Upon photoexcitation, all six complexes in fluid CH2Cl2 and aqueous solutions at room temperature displayed intense—with quantum yields as high as 0.60 (complex 4b)—and quite long-lived blue-green luminescence, corresponding in each case to a vibronically structured emission profile peaking at ca. 480 and ca. 508 nm. These features, in combination with the reduced rigidochromic shift that was observed from the same samples frozen at 77 K, suggested the 3LC-type excited states as the prevalent contributors to the emission. The interactions of these new biotinylated complexes with avidin have been studied by 4′-hydroxyazobenzene-2-carboxylic acid (HABA) assays and emission titrations. In general, the amplification of the emission intensity of the Ir(III) complexes occurring upon their interaction with avidin was observed. It is also worth mentioning how a similar or better response was displayed by [(C∧N)2Ir(N∧N-spacer-O-CO-biotin)]+-type complexes, in which biotin is appended to the Ir(III) lumophore through an ester moiety.
Showing items related by title, author, creator and subject.
Vaughan, J.; Reid, Brodie; Wright, Phillip; Ramchandani, S.; Skelton, B.; Raiteri, Paolo; Muzzioli, S.; Brown, David; Stagni, S.; Massi, Massimiliano (2014)A family of tricarbonyl Re(I) complexes of the formulation fac-[Re(CO)3(NHC)L] has been synthesized and characterized, both spectroscopically and structurally. The NHC ligand represents a bidentate N-heterocyclic carbene ...
Gullberg, B.; De Breuck, C.; Lehnert, M.; Vernet, J.; Bacon, R.; Drouart, Guillaume; Emonts, B.; Galametz, A.; Ivison, R.; Nesvadba, N.; Richard, J.; Seymour, Nick; Stern, D.; Wylezalek, D. (2016)© 2016 ESO. We present a pilot study of the z = 2.923 radio galaxy MRC0943-242, where we combine information from ALMA and MUSE data cubes for the first time. Even with modest integration times, we disentangle the AGN and ...
Crystal structure, spectroscopy and crystal field analysis of substituted 1,10-phenanthroline-europium complexesPan, Z.; Jia, Guohua; Duan, C.; Wong, W.; Wong, W.; Tanner, P. (2011)Crystal structures and vibrational and electronic spectroscopic data are reported for europium(III) complexes with 5(or 4)-R-1,10-phenanthroline (phen) ligands (R = chloro, methyl, nitro, amino). All complexes comprise a ...