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    A fluorescence-based quantitative real-time PCR assay for accurate Pocillopora damicornis species identification

    Access Status
    Fulltext not available
    Authors
    Thomas, L.
    Stat, Michael
    Evans, R.
    Kennington, W.
    Date
    2016
    Type
    Journal Article
    
    Metadata
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    Citation
    Thomas, L. and Stat, M. and Evans, R. and Kennington, W. 2016. A fluorescence-based quantitative real-time PCR assay for accurate Pocillopora damicornis species identification. Coral Reefs. 35 (3): pp. 895-899.
    Source Title
    Coral Reefs, International Society for Reef Studies
    DOI
    10.1007/s00338-016-1430-3
    ISSN
    0722-4028
    School
    Department of Environment and Agriculture
    URI
    http://hdl.handle.net/20.500.11937/38378
    Collection
    • Curtin Research Publications
    Abstract

    Pocillopora damicornis is one of the most extensively studied coral species globally, but high levels of phenotypic plasticity within the genus make species identification based on morphology alone unreliable. As a result, there is a compelling need to develop cheap and time-effective molecular techniques capable of accurately distinguishing P. damicornis from other congeneric species. Here, we develop a fluorescence-based quantitative real-time PCR (qPCR) assay to genotype a single nucleotide polymorphism that accurately distinguishes P. damicornis from other morphologically similar Pocillopora species. We trial the assay across colonies representing multiple Pocillopora species and then apply the assay to screen samples of Pocillopora spp. collected at regional scales along the coastline of Western Australia. This assay offers a cheap and time-effective alternative to Sanger sequencing and has broad applications including studies on gene flow, dispersal, recruitment and physiological thresholds of P. damicornis.

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