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dc.contributor.authorMurray, D.
dc.contributor.authorPearse, S.
dc.contributor.authorFullagar, R.
dc.contributor.authorChase, B.
dc.contributor.authorHouston, J.
dc.contributor.authorAtchison, J.
dc.contributor.authorWhite, Nicole
dc.contributor.authorBellgard, M.
dc.contributor.authorClarke, E.
dc.contributor.authorMacphail, M.
dc.contributor.authorGilbert, Thomas
dc.contributor.authorHaile, James
dc.contributor.authorBunce, Michael
dc.date.accessioned2017-01-30T14:29:04Z
dc.date.available2017-01-30T14:29:04Z
dc.date.created2014-11-19T01:13:36Z
dc.date.issued2012
dc.identifier.citationMurray, D. and Pearse, S. and Fullagar, R. and Chase, B. and Houston, J. and Atchison, J. and White, N. et al. 2012. High-throughput sequencing of ancient plant and mammal DNA preserved in herbivore middens. Quaternary Science Reviews. 58: pp. 135-145.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/39016
dc.identifier.doi10.1016/j.quascirev.2012.10.021
dc.description.abstract

The study of arid palaeoenvironments is often frustrated by the poor or non-existent preservation ofplant and animal material, yet these environments are of considerable environmental importance. Theanalysis of pollen and macrofossils isolated from herbivore middens has been an invaluable source ofinformation regarding past environments and the nature of ecological fluctuations within arid zones. Theapplication of ancient DNA (aDNA) techniques to hot, arid zone middens remains unexplored. This paperattempts to retrieve and characterise aDNA from four Southern Hemisphere fossil middens; three locatedin hot, arid regions of Australia and one sample from South Africa’s Western Cape province. The middensare dated to between 30,490 (!380) and 710 (!70) cal yr BP. The Brockman Ridge midden in this studyis potentially the oldest sample from which aDNA has been successfully extracted in Australia. Theapplication of high-throughput sequencing approaches to profile the biotic remains preserved in middenmaterial has not been attempted to date and this study clearly demonstrates the potential of sucha methodology. In addition to the taxa previously detected via macrofossil and palynological analyses,aDNA analysis identified unreported plant and animal taxa, some of which are locally extinct or endemic.The survival and preservation of DNA in hot, arid environments is a complex and poorly understoodprocess that is both sporadic and rare, but the survival of DNA through desiccation may be important.Herbivore middens now present an important source of material for DNA metabarcoding studies of hot,arid palaeoenvironments and can potentially be used to analyse middens in these environmentsthroughout Australia, Africa, the Americas and the Middle East.

dc.publisherPergamon
dc.subjectArid zone
dc.subjectHerbivore midden
dc.subjectPalaeoenvironment
dc.subjectAncient DNA
dc.subjectMetabarcoding - High throughput sequencing
dc.titleHigh-throughput sequencing of ancient plant and mammal DNA preserved in herbivore middens
dc.typeJournal Article
dcterms.source.volume58
dcterms.source.startPage135
dcterms.source.endPage145
dcterms.source.issn0277-3791
dcterms.source.titleQuaternary Science Reviews
curtin.accessStatusFulltext not available


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