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dc.contributor.authorFarman, M.
dc.contributor.authorOliver, Richard
dc.date.accessioned2017-01-30T14:29:16Z
dc.date.available2017-01-30T14:29:16Z
dc.date.created2010-11-12T04:17:01Z
dc.date.issued1988
dc.identifier.citationFARMAN ML & OLIVER RP (1988) The transformation of protoplasts of Leptosphaeria maculans to Hygromycin B resistance. Current Genetics 13 327-330
dc.identifier.urihttp://hdl.handle.net/20.500.11937/39036
dc.identifier.doi10.1007/BF00424427
dc.description.abstract

Conditions are described for the efficient isolation and regeneration of protoplasts of a fungal pathogen of brassicas, Leptosphaeria maculans. Treatment of the protoplasts with DNA of the plasmid pAN7-1 (containing an E. coli hygromycin phosphotransferase gene with Aspergillus nidulans expression signals) and plating under selective conditions resulted in the formation of hygromycin 13-resistant colonies. Southern blot analysis of resistant colonies indicated that single copies of the plasmid had integrated into different sites in the genome. In twelve of the transformants analysed so far, the integration is stable through mitosis. The demonstration of efficient transformation is an essential first step in the molecular analysis of pathogenicity of this commercially important pathogen.

dc.titleThe transformation of protoplasts of Leptosphaeria maculans to Hygromycin B resistance
dc.typeJournal Article
curtin.note

A copy of this item may be available from Professor Richard Oliver

curtin.note

Email: Richard.oliver@curtin.edu.au

curtin.accessStatusFulltext not available
curtin.facultyDepartment of Environmental & Agriculture
curtin.facultySchool of Agriculture and Environment
curtin.facultyFaculty of Science and Engineering


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