The transformation of protoplasts of Leptosphaeria maculans to Hygromycin B resistance
dc.contributor.author | Farman, M. | |
dc.contributor.author | Oliver, Richard | |
dc.date.accessioned | 2017-01-30T14:29:16Z | |
dc.date.available | 2017-01-30T14:29:16Z | |
dc.date.created | 2010-11-12T04:17:01Z | |
dc.date.issued | 1988 | |
dc.identifier.citation | FARMAN ML & OLIVER RP (1988) The transformation of protoplasts of Leptosphaeria maculans to Hygromycin B resistance. Current Genetics 13 327-330 | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/39036 | |
dc.identifier.doi | 10.1007/BF00424427 | |
dc.description.abstract |
Conditions are described for the efficient isolation and regeneration of protoplasts of a fungal pathogen of brassicas, Leptosphaeria maculans. Treatment of the protoplasts with DNA of the plasmid pAN7-1 (containing an E. coli hygromycin phosphotransferase gene with Aspergillus nidulans expression signals) and plating under selective conditions resulted in the formation of hygromycin 13-resistant colonies. Southern blot analysis of resistant colonies indicated that single copies of the plasmid had integrated into different sites in the genome. In twelve of the transformants analysed so far, the integration is stable through mitosis. The demonstration of efficient transformation is an essential first step in the molecular analysis of pathogenicity of this commercially important pathogen. | |
dc.title | The transformation of protoplasts of Leptosphaeria maculans to Hygromycin B resistance | |
dc.type | Journal Article | |
curtin.note |
A copy of this item may be available from Professor Richard Oliver | |
curtin.note |
Email: Richard.oliver@curtin.edu.au | |
curtin.accessStatus | Fulltext not available | |
curtin.faculty | Department of Environmental & Agriculture | |
curtin.faculty | School of Agriculture and Environment | |
curtin.faculty | Faculty of Science and Engineering |