Whole-genome sequencing and social-network analysis of a tuberculosis outbreak
dc.contributor.author | Gardy, J. | |
dc.contributor.author | Johnston, J. | |
dc.contributor.author | Ho Sui, S. | |
dc.contributor.author | Cook, V. | |
dc.contributor.author | Shah, L. | |
dc.contributor.author | Brodkin, E. | |
dc.contributor.author | Rempel, S. | |
dc.contributor.author | Moore, R. | |
dc.contributor.author | Zhao, Y. | |
dc.contributor.author | Holt, R. | |
dc.contributor.author | Varhol, Richard | |
dc.contributor.author | Birol, I. | |
dc.contributor.author | Lem, M. | |
dc.contributor.author | Sharma, M. | |
dc.contributor.author | Elwood, K. | |
dc.contributor.author | Jones, S. | |
dc.contributor.author | Brinkman, F. | |
dc.contributor.author | Brunham, R. | |
dc.contributor.author | Tang, P. | |
dc.date.accessioned | 2017-01-30T14:30:00Z | |
dc.date.available | 2017-01-30T14:30:00Z | |
dc.date.created | 2016-02-01T00:47:10Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Gardy, J. and Johnston, J. and Ho Sui, S. and Cook, V. and Shah, L. and Brodkin, E. and Rempel, S. et al. 2011. Whole-genome sequencing and social-network analysis of a tuberculosis outbreak. New England Journal of Medicine. 364 (8): pp. 730-739. | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/39071 | |
dc.identifier.doi | 10.1056/NEJMoa1003176 | |
dc.description.abstract |
Background: An outbreak of tuberculosis occurred over a 3-year period in a medium-size community in British Columbia, Canada. The results of mycobacterial interspersed repetitive unit-variable-number tandem-repeat (MIRU-VNTR) genotyping suggested the outbreak was clonal. Traditional contact tracing did not identify a source. We used whole-genome sequencing and social-network analysis in an effort to describe the outbreak dynamics at a higher resolution. Methods: We sequenced the complete genomes of 32 Mycobacterium tuberculosis outbreak isolates and 4 historical isolates (from the same region but sampled before the outbreak) with matching genotypes, using short-read sequencing. Epidemiologic and genomic data were overlaid on a social network constructed by means of interviews with patients to determine the origins and transmission dynamics of the outbreak. Results: Whole-genome data revealed two genetically distinct lineages of M. tuberculosis with identical MIRU-VNTR genotypes, suggesting two concomitant outbreaks. Integration of social-network and phylogenetic analyses revealed several transmission events, including those involving "superspreaders." Both lineages descended from a common ancestor and had been detected in the community before the outbreak, suggesting a social, rather than genetic, trigger. Further epidemiologic investigation revealed that the onset of the outbreak coincided with a recorded increase in crack cocaine use in the community. Conclusions: Through integration of large-scale bacterial whole-genome sequencing and social-network analysis, we show that a socioenvironmental factor - most likely increased crack cocaine use - triggered the simultaneous expansion of two extant lineages of M. tuberculosis that was sustained by key members of a high-risk social network. Genotyping and contact tracing alone did not capture the true dynamics of the outbreak. (Funded by Genome British Columbia and others.) Copyright © 2011 Massachusetts Medical Society. | |
dc.title | Whole-genome sequencing and social-network analysis of a tuberculosis outbreak | |
dc.type | Journal Article | |
dcterms.source.volume | 364 | |
dcterms.source.number | 8 | |
dcterms.source.startPage | 730 | |
dcterms.source.endPage | 739 | |
dcterms.source.issn | 0028-4793 | |
dcterms.source.title | New England Journal of Medicine | |
curtin.department | Department of Health Policy and Management | |
curtin.accessStatus | Fulltext not available |
Files in this item
Files | Size | Format | View |
---|---|---|---|
There are no files associated with this item. |