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dc.contributor.authorGogoi Tiwari, Jully
dc.contributor.authorBabra Waryah, C.
dc.contributor.authorSunagar, R.
dc.contributor.authorVeeresh, H.
dc.contributor.authorNuthanalakshmi, V.
dc.contributor.authorPreethirani, P.
dc.contributor.authorSharada, R.
dc.contributor.authorIsloor, S.
dc.contributor.authorBhat, A.
dc.contributor.authorAl-Salami, Hani
dc.contributor.authorHegde, N.
dc.contributor.authorMukkur, T.
dc.date.accessioned2017-01-30T14:45:03Z
dc.date.available2017-01-30T14:45:03Z
dc.date.created2015-10-29T04:08:53Z
dc.date.issued2015
dc.identifier.citationGogoi Tiwari, J. and Babra Waryah, C. and Sunagar, R. and Veeresh, H. and Nuthanalakshmi, V. and Preethirani, P. and Sharada, R. et al. 2015. Typing of Staphylococcus aureus isolated from bovine mastitis cases in Australia and India. Australian Veterinary Journal. 93 (8): pp. 278-282.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/40722
dc.identifier.doi10.1111/avj.12349
dc.description.abstract

Objective: To determine the prevalence of the different capsular polysaccharide (CP) and major surface-associated non-CP antigen 336 (SP-336) types among Staphylococcus aureus isolated from bovine mastitis cases in Australia and India. Methods: A total of 414 strains (154 from Australia, 260 from India) isolated from clinical bovine mastitis were included in the study. Mouse antisera raised against CP types (CP1, CP2, CP5, and CP8) or SP-336 were used in slide agglutination tests and compared with detection of cap1, cap5 and cap8 gene fragments by PCR. Results: Serological studies revealed the presence of CP2, CP5, CP8 and SP-336 in 9.1%, 23.4%, 31.8%, and 5.8% of the Australian versus 0.8%, 46.9%, 13.1% and 0% of the Indian isolates, respectively. By PCR, CP1, CP5 and CP8 accounted for 0%, 26.6% and 32.4% of the Australian versus 3.9%, 85% and 8.1% of the Indian isolates, respectively. Conclusions: Both PCR and the serological method demonstrated that CP5 and CP8 are the predominant capsular types in Australia, whereas CP5 is the predominant capsular type in India. The study also demonstrated a strong correlation between both methods of typing for CP1, CP5, CP8 and non-typeable S. aureus strains. High-percentage prevalence of non-typeable isolates in both the countries highlights the importance of continued investigations of the identification of unique surface-associated polysaccharide antigens prevalent among S. aureus isolates for the formulation of CP- and SP-based vaccines for bovine mastitis.

dc.publisherBlackwell Publishing
dc.titleTyping of Staphylococcus aureus isolated from bovine mastitis cases in Australia and India
dc.typeJournal Article
dcterms.source.volume93
dcterms.source.number8
dcterms.source.startPage278
dcterms.source.endPage282
dcterms.source.issn0005-0423
dcterms.source.titleAustralian Veterinary Journal
curtin.note

This is the peer reviewed version of the following article:Gogoi Tiwari, J. and Babra Waryah, C. and Sunagar, R. and Veeresh, H. and Nuthanalakshmi, V. and Preethirani, P. and Sharada, R. et al. 2015. Typing of Staphylococcus aureus isolated from bovine mastitis cases in Australia and India. Australian Veterinary Journal. 93 (8): pp. 278-282.,which has been published in final form at http://doi.org/10.1111/avj.12349This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving at http://olabout.wiley.com/WileyCDA/Section/id-820227.html#terms

curtin.departmentSchool of Pharmacy
curtin.accessStatusOpen access


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