A rapid non-destructive DNA extraction method for insects and other arthropods
MetadataShow full item record
Preparation of arthropods for morphological identification often damages or destroys DNA within the specimen. Conversely, DNA extraction methods often destroy the external physical characteristics essential for morphological identification. We have developed a rapid, simple and non-destructive DNA extraction technique for arthropod specimens. This technique was tested on four arthropod orders, using specimens that were fresh, preserved by air drying, stored in ethanol, or collected with sticky or propylene glycol traps. The technique could be completed in twenty minutes for Coleoptera, Diptera and Hemiptera, and two minutes for the subclass Acarina, without significant distortion, discolouration, or other damage to the specimens.
The link to the journal’s home page is: http://www.elsevier.com/wps/find/journaldescription.cws_home/715159/description#description. Copyright © 2010 Elsevier B.V. All rights reserved
Showing items related by title, author, creator and subject.
Oil mallee plantings and arthropod biodiversity in the Western Australian wheatbelt : effects of host species, nutrition, and leaf chemistryLyons, Anita Marie (2008)Since European settlement, around 93% of the Western Australian wheatbelt has been cleared for agriculture, leading to a range of environmental problems, including erosion, salinity, and loss of biodiversity. Recently, ...
Biology and systematics of Trogoderma species with special reference to morphological and molecular diagnostic techniques for identification of Trogoderma pest speciesCastalanelli, Mark A. (2011)The genus Trogoderma contains some of the world’s most serious invasive pests of wheat and other stored grain products. Even with the application of strict quarantine measures, these grain beetles still find their way ...
Arthropods in coarse woody debris in jarrah forest and rehabilitated bauxite mines in Western AustraliaKoch, John; Grigg, A.; Gordon, Ross; Majer, Jonathan (2010)Coarse woody debris (CWD) is returned to Alcoa’s rehabilitated mined areas in the jarrah forest as potential vertebrate fauna habitat, however, its value for invertebrate fauna has not been investigated. We sought to ...