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    A rapid non-destructive DNA extraction method for insects and other arthropods

    142529_142529.pdf (169.6Kb)
    Access Status
    Open access
    Authors
    Castalanelli, M.
    Severtson, D.
    Brumley, C.
    Szito, A.
    Foottit, R.
    Grimm, M.
    Munyard, Kylie
    Groth, David
    Date
    2010
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Castalanelli, Mark and Severtson, Dustin and Brumley, Cameron and Szito, Andras and Foottit, Robert and Grimm, Michael and Munyard, Kylie and Groth, David. 2010. A rapid non-destructive DNA extraction method for insects and other arthropods. Journal of Asia-Pacific Entomology. 13 (3): pp. 243-248.
    Source Title
    Journal of Asia-Pacific Entomology
    DOI
    10.1016/j.aspen.2010.04.003
    ISSN
    12268615
    Faculty
    Faculty of Health Sciences
    School of Biomedical Sciences
    Remarks

    The link to the journal’s home page is: http://www.elsevier.com/wps/find/journaldescription.cws_home/715159/description#description. Copyright © 2010 Elsevier B.V. All rights reserved

    URI
    http://hdl.handle.net/20.500.11937/40898
    Collection
    • Curtin Research Publications
    Abstract

    Preparation of arthropods for morphological identification often damages or destroys DNA within the specimen. Conversely, DNA extraction methods often destroy the external physical characteristics essential for morphological identification. We have developed a rapid, simple and non-destructive DNA extraction technique for arthropod specimens. This technique was tested on four arthropod orders, using specimens that were fresh, preserved by air drying, stored in ethanol, or collected with sticky or propylene glycol traps. The technique could be completed in twenty minutes for Coleoptera, Diptera and Hemiptera, and two minutes for the subclass Acarina, without significant distortion, discolouration, or other damage to the specimens.

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