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    Synergistic effects of high fat feeding and apolipoprotein E deletion on enterocytic amyloid-beta abundance.

    117169_7153_2008_ apo E_LHD.pdf (596.9Kb)
    Access Status
    Open access
    Authors
    Galloway, Susan
    Pallebage-Gamarallage, Menuka
    Takechi, Ryusuke
    Jian, Le
    Johnsen , Russell
    Dhaliwal, Satvinder
    Mamo, John
    Date
    2008
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Galloway, Susan and Pallebage-Gamarallage, Menuka and Takechi, Ryusuke and Jian, Le and Johnsen, Russell and Dhaliwal, Satvinder and Mamo, John. 2008. Synergistic effects of high fat feeding and apolipoprotein E deletion on enterocytic amyloid-beta abundance. Lipids in Health and Disease. 7 (15).
    Source Title
    Lipids in Health and Disease
    DOI
    10.1186/1476-511X-7-15
    ISSN
    1476-511X
    Faculty
    Faculty of Health Sciences
    School of Public Health
    Remarks

    This article is published under the Open Access publishing model and distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/ Please refer to the licence to obtain terms for any further reuse or distribution of this work.

    URI
    http://hdl.handle.net/20.500.11937/41788
    Collection
    • Curtin Research Publications
    Abstract

    Background: Amyloid-β (Aβ), a key protein found in amyloid plaques of subjects with Alzheimer's disease is expressed in the absorptive epithelial cells of the small intestine. Ingestion of saturated fat significantly enhances enterocytic Aβ abundance whereas fasting abolishes expression. Apolipoprotein (apo) E has been shown to directly modulate Aβ biogenesis in liver and neuronal cells but it's effect in enterocytes is not known. In addition, apo E modulates villi length, which may indirectly modulate Aβ as a consequence of differences in lipid absorption. This study compared Aβ abundance and villi length in wild-type (WT) and apo E knockout (KO) mice maintained on either a low-fat or high-fat diet. Wild-type C57BL/6J and apo E KO mice were randomised for six-months to a diet containing either 4% (w/w) unsaturated fats, or chow comprising 16% saturated fats and 1% cholesterol. Quantitative immunohistochemistry was used to assess Aβ abundance in small intestinal enterocytes. Apo E KO mice given the low-fat diet had similar enterocytic Aβ abundance compared to WT controls. Results: The saturated fat diet substantially increased enterocytic Aβ in WT and in apo E KO mice, however the effect was greater in the latter. Villi height was significantly greater in apo E KO mice than for WT controls when given the low-fat diet. However, WT mice had comparable villi length to apo E KO when fed the saturated fat and cholesterol enriched diet. There was no effect of the high-fat diet on villi length in apo E KO mice. Conclusion: The findings of this study are consistent with the notion that lipid substrate availability modulates enterocytic Aβ. Apo E may influence enterocytic lipid availability by modulating absorptive capacity.

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